Ecteinascidin 743, a transcription-targeted chemotherapeutic that inhibits MDR1 activation Journal Article
| Authors: | Jin, S.; Gorfajn, B.; Faircloth, G.; Scotto, K. W. |
| Article Title: | Ecteinascidin 743, a transcription-targeted chemotherapeutic that inhibits MDR1 activation |
| Abstract: | Ecteinascidin 743 (ET-743), a highly promising marine-based antitumor agent presently in phase II clinical trials, has been shown to interfere with the binding of minor-groove-interacting transcription factors, particularly NF-Y, with their cognate promoter elements in vitro. We have shown that NF-Y is a central mediator of activation of transcription of the human P glycoprotein gene (MDR1) by a variety of inducers and that NF-Y functions by recruiting the histone acetyltransferase PCAF to the MDR1 promoter. In the present study, we tested whether ET-743 could block activation of the MDR1 promoter by agents that mediate their effect through the NF-Y/PCAF complex. We report that physiologically relevant concentrations of ET-743 abrogate transcriptional activation of both the endogenous MDR1 gene and MDR1 reporter constructs by the histone deacetylase inhibitors as well as by UV light, with minimal effect on constitutive MDR1 transcription. Notably, this inhibition does not alter the promoter-associated histone hyperacetylation induced by histone deacetylase inhibitors, suggesting an in vivo molecular target downstream of NF-Y/PCAF binding. ET-743 is therefore the prototype for a distinct class of transcription-targeted chemotherapeutic agents and may be an efficacious adjuvant to the treatment of multidrug-resistant tumors. |
| Keywords: | controlled study; human cell; promoter region; dna-binding proteins; clinical trial; antineoplastic agent; cell cycle; controlled clinical trial; cancer cell culture; drug potency; tumor cells, cultured; gene activation; dna; transcription regulation; rna, messenger; antineoplastic agents, alkylating; hydroxamic acids; trabectedin; colon carcinoma; drug binding; p-glycoprotein; ccaat-enhancer-binding proteins; acetylation; dioxoles; isoquinolines; promoter regions (genetics); tetrahydroisoquinolines; humans; human; priority journal; article |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 97 |
| Issue: | 12 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2000-06-06 |
| Start Page: | 6775 |
| End Page: | 6779 |
| Language: | English |
| DOI: | 10.1073/pnas.97.12.6775 |
| PUBMED: | 10841572 |
| PROVIDER: | scopus |
| PMCID: | PMC18735 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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