Monitoring CD8 T cell responses to NY-ESO-1: Correlation of humoral and cellular immune responses Journal Article


Authors: Jäger, E.; Nagata, Y.; Gnjatic, S.; Wada, H.; Stockert, E.; Karbach, J.; Dunbar, P. R.; Lee, S. Y.; Jungbluth, A.; Jäger, D.; Arand, M.; Ritter, G.; Cerundolo, V.; Dupont, B.; Chen, Y. T.; Old, L. J.; Knuth, A.
Article Title: Monitoring CD8 T cell responses to NY-ESO-1: Correlation of humoral and cellular immune responses
Abstract: NY-ESO-1, a member of the cancer-testis family of antigens, is expressed in a subset of a broad range of different human tumor types. Patients with advanced NY-ESO-1-expressing tumors frequently develop humoral immunity to NY-ESO-1, and three HLA A2-restricted peptides were defined previously as targets for cytotoxic CD8+ T cells in a melanoma patient with NY-ESO-1 antibody. The objectives of the present study were (i) to develop enzyme- linked immunospot (ELISPOT) and tetramer assays to measure CD8+ T cell responses to NY-ESO-1, (ii) to determine the frequency of CD8+ T cell responses to NY-ESO-1 in a series of HLA-A2 patients with NY-ESO-1 expressing tumors, (ii) to determine the relation between CD8+ T cell and humoral immune responses to NY-ESO-1, and (iv) to compare results of NY-ESO-1 ELISPOT assays performed independently in two laboratories with T cells from the same patients. NY-ESO-1 ELISPOT and tetramer assays with excellent sensitivity, specificity, and reproducibility have been developed and found to correlate with cytotoxicity assays. CD8+ T cell responses to HLA-A2-restricted NY-ESO- 1 peptides were detected in 10 of 11 patients with NY-ESO-1 antibody, but not in patients lacking antibody or in patients with NY-ESO-1-negative tumors. The results of ELISPOT assays were concordant in the two laboratories, providing the basis for standardized monitoring of T cell responses in patients receiving NY-ESO-1 vaccines.
Keywords: cd8 antigen; cd8-positive t-lymphocytes; reproducibility; proteins; cells, cultured; melanoma; membrane proteins; cytotoxicity; enzyme activity; immunoreactivity; cellular immunity; immune response; antigens, neoplasm; rna, messenger; peptide fragments; cytotoxicity, immunologic; enzyme-linked immunosorbent assay; antigen binding; humoral immunity; antibody formation; hla-a2 antigen; immunity, cellular; histocompatibility testing; humans; priority journal; article
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 97
Issue: 9
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2000-04-25
Start Page: 4760
End Page: 4765
Language: English
DOI: 10.1073/pnas.97.9.4760
PUBMED: 10781081
PROVIDER: scopus
PMCID: PMC18306
DOI/URL:
Notes: Export Date: 18 November 2015 -- Source: Scopus
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MSK Authors
  1. Yasuhiro Nagata
    11 Nagata
  2. Sang Yull Lee
    16 Lee
  3. Sacha Gnjatic
    111 Gnjatic
  4. Achim Jungbluth
    341 Jungbluth
  5. Gerd Ritter
    158 Ritter
  6. Bo Dupont
    156 Dupont
  7. Lloyd J Old
    385 Old