Monitoring CD8 T cell responses to NY-ESO-1: Correlation of humoral and cellular immune responses Journal Article
| Authors: | Jäger, E.; Nagata, Y.; Gnjatic, S.; Wada, H.; Stockert, E.; Karbach, J.; Dunbar, P. R.; Lee, S. Y.; Jungbluth, A.; Jäger, D.; Arand, M.; Ritter, G.; Cerundolo, V.; Dupont, B.; Chen, Y. T.; Old, L. J.; Knuth, A. |
| Article Title: | Monitoring CD8 T cell responses to NY-ESO-1: Correlation of humoral and cellular immune responses |
| Abstract: | NY-ESO-1, a member of the cancer-testis family of antigens, is expressed in a subset of a broad range of different human tumor types. Patients with advanced NY-ESO-1-expressing tumors frequently develop humoral immunity to NY-ESO-1, and three HLA A2-restricted peptides were defined previously as targets for cytotoxic CD8+ T cells in a melanoma patient with NY-ESO-1 antibody. The objectives of the present study were (i) to develop enzyme- linked immunospot (ELISPOT) and tetramer assays to measure CD8+ T cell responses to NY-ESO-1, (ii) to determine the frequency of CD8+ T cell responses to NY-ESO-1 in a series of HLA-A2 patients with NY-ESO-1 expressing tumors, (ii) to determine the relation between CD8+ T cell and humoral immune responses to NY-ESO-1, and (iv) to compare results of NY-ESO-1 ELISPOT assays performed independently in two laboratories with T cells from the same patients. NY-ESO-1 ELISPOT and tetramer assays with excellent sensitivity, specificity, and reproducibility have been developed and found to correlate with cytotoxicity assays. CD8+ T cell responses to HLA-A2-restricted NY-ESO- 1 peptides were detected in 10 of 11 patients with NY-ESO-1 antibody, but not in patients lacking antibody or in patients with NY-ESO-1-negative tumors. The results of ELISPOT assays were concordant in the two laboratories, providing the basis for standardized monitoring of T cell responses in patients receiving NY-ESO-1 vaccines. |
| Keywords: | cd8 antigen; cd8-positive t-lymphocytes; reproducibility; proteins; cells, cultured; melanoma; membrane proteins; cytotoxicity; enzyme activity; immunoreactivity; cellular immunity; immune response; antigens, neoplasm; rna, messenger; peptide fragments; cytotoxicity, immunologic; enzyme-linked immunosorbent assay; antigen binding; humoral immunity; antibody formation; hla-a2 antigen; immunity, cellular; histocompatibility testing; humans; priority journal; article |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 97 |
| Issue: | 9 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2000-04-25 |
| Start Page: | 4760 |
| End Page: | 4765 |
| Language: | English |
| DOI: | 10.1073/pnas.97.9.4760 |
| PUBMED: | 10781081 |
| PROVIDER: | scopus |
| PMCID: | PMC18306 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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