The identification of an endonuclease that cleaves within an HuR binding site in mRNA Journal Article
| Authors: | Zhao, Z.; Chang, F. C.; Furneaux, H. M. |
| Article Title: | The identification of an endonuclease that cleaves within an HuR binding site in mRNA |
| Abstract: | Messenger RNAs (mRNAs) that contain U-rich elements are targeted for rapid decay. Selective inhibition of this decay results in a rapid increase in steady state level. Thus, this is an important regulatory step in gene expression. Previously, we have found that these mRNAs are selectively stabilized by a specific mRNA binding protein called HuR. mechanism of action of HuR is not well understood. It has been postulated that HuR stabilizes mRNA by the displacement or inhibition of factors that specifically cleave or deadenylate these mRNAs. In this paper, we report the identification and characterization of a novel endonuclease that cleaves within an HuR binding site in p27kip1 mRNA. The specificity of this endonuclease and its inhibition by HuR argue for it playing a role in the postranscriptional regulation of gene expression. |
| Keywords: | controlled study; human cell; enzyme inhibition; steady state; hela cells; rna binding protein; gene expression regulation; rna-binding proteins; transcription regulation; messenger rna; enzyme analysis; rna, messenger; eukaryota; substrate specificity; genetic stability; base sequence; binding site; binding sites; endonuclease; enzyme specificity; rna cleavage; antigens, surface; uracil; endonucleases; decomposition; humans; human; priority journal; article |
| Journal Title: | Nucleic Acids Research |
| Volume: | 28 |
| Issue: | 14 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 2000-07-15 |
| Start Page: | 2695 |
| End Page: | 2701 |
| Language: | English |
| PUBMED: | 10908325 |
| PROVIDER: | scopus |
| PMCID: | PMC102663 |
| DOI: | 10.1093/nar/28.14.2695 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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