Assembly of archaeal signal recognition particle from recombinant components Journal Article
| Authors: | Bhuiyan, S. H.; Gowda, K.; Hotokezaka, H.; Zwieb, C. |
| Article Title: | Assembly of archaeal signal recognition particle from recombinant components |
| Abstract: | Signal recognition particle (SRP) takes part in protein targeting and secretion in all organisms. Searches for components of archaeal SRP in primary databases and completed genomes indicated that archaea possess only homologs of SRP RNA, and proteins SRP19 and SRP54. A recombinant SRP was assembled from cloned, expressed and purified components of the hyperthermophilic archaeon Archaeoglobus fulgidus. Recombinant Af-SRP54 associated with the signal peptide of bovine preprolactin translated in vitro.As in mammalian SRP, Af-SRP54 binding to Af-SRP RNA required protein Af-SRP19, although notable amounts bound in absence of Af-SRP19. Archaeoglobus fulgidus SRP proteins also bound to full-length SRP RNA of the archaeon Methanococcus jannaschii, to eukaryotic human SRP RNA, and to truncated versions which corresponded to the large domain of SRP. Dependence on SRP19 was most pronounced with components from the same species. Reconstitutions with heterologous components revealed a significant potential of human SRP proteins to bind to archaeal SRP RNAs. Surprisingly, M. jannaschii SRP RNA bound to human SRP54M quantitatively in the absence of SRP19. This is the first report of reconstitution of an archaeal SRP from recombinantly expressed purified components. The results highlight structural and functional conservation of SRP assembly between archaea and eucarya. |
| Keywords: | nonhuman; mammalia; animals; protein targeting; protein binding; data base; bacteria (microorganisms); rna; molecular cloning; cloning, molecular; rna-binding proteins; conserved sequence; molecular sequence data; protein purification; eukaryota; molecular recognition; recombinant proteins; recombinant protein; protein secretion; protein biosynthesis; base sequence; cattle; saccharomyces cerevisiae proteins; nucleic acid conformation; archaebacterium; archaea; prolactin; protein precursors; cross-linking reagents; bovinae; bacterial rna; protein sorting signals; archaeoglobus fulgidus; signal recognition particle; humans; priority journal; article; methanococcus jannaschii; methanococcus; methanocaldococcus jannaschii; azirines |
| Journal Title: | Nucleic Acids Research |
| Volume: | 28 |
| Issue: | 6 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 2000-03-15 |
| Start Page: | 1365 |
| End Page: | 1373 |
| Language: | English |
| PUBMED: | 10684931 |
| PROVIDER: | scopus |
| PMCID: | PMC111052 |
| DOI: | 10.1093/nar/28.6.1365 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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