Identification of a unique domain essential for Escherichia coli DNA topoisomerase III-catalysed decatenation of replication intermediates Journal Article
| Authors: | Li, Z.; Mondragón, A.; Hiasa, H.; Marians, K. J.; DiGate, R. J. |
| Article Title: | Identification of a unique domain essential for Escherichia coli DNA topoisomerase III-catalysed decatenation of replication intermediates |
| Abstract: | A 17-amino-acid residue domain has been identified in Escherichia coli DNA topoisomerase II (Topo III) that is essential for Topo III-mediated resolution of DNA replication intermediates in vitro. Deletion of this domain reduced Topo III-catalysed resolution of DNA replication intermediates and decatenation of multiply linked plasmid DNA dimers by four orders of magnitude, whereas reducing Topo III-catalysed relaxation of negatively supercoiled DNA substrates only 20-fold. The presence of this domain has been detected in multiple plasmid-encoded topoisomerases, raising the possibility that these enzymes may also be decatenases. |
| Keywords: | controlled study; nonhuman; dna replication; protein domain; protein binding; bacteria (microorganisms); amino acid sequence; molecular sequence data; sequence homology, amino acid; recombinant fusion proteins; escherichia coli; substrate specificity; dimerization; protein structure, tertiary; binding sites; nucleic acid conformation; dna, bacterial; dna topoisomerase; dna topoisomerases, type i; plasmid dna; dna supercoiling; dna, superhelical; dimer; negibacteria; priority journal; article |
| Journal Title: | Molecular Microbiology |
| Volume: | 35 |
| Issue: | 4 |
| ISSN: | 0950-382X |
| Publisher: | Blackwell Publishing |
| Date Published: | 2000-02-01 |
| Start Page: | 888 |
| End Page: | 895 |
| Language: | English |
| DOI: | 10.1046/j.1365-2958.2000.01763.x |
| PUBMED: | 10692165 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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