Chemotaxis of primitive hematopoietic cells in response to stromal cell-derived factor-1 Journal Article
| Authors: | Jo, D. Y.; Rafii, S.; Hamada, T.; Moore, M. A. S. |
| Article Title: | Chemotaxis of primitive hematopoietic cells in response to stromal cell-derived factor-1 |
| Abstract: | Stromal cell-derived factor-1 (SDF-1) provides a potent chemotactic stimulus for CD34+ hematopoietic cells. We cultured mobilized peripheral blood (PB) and umbilical cord blood (CB) for up to 5 weeks and examined the migratory activity of cobblestone area-forming cells (CAFCs) and long-term culture-initiating cells (LTC-ICs) in a transwell assay. In this system, SDF- 1 or MS-5 marrow stromal cells placed in the lower chamber induced transmembrane and transendothelial migration by 2- and 5-week-old CAFCs and LTC-ICs in 3 hours. Transmigration was blocked by preincubation of input CD34+ cells with antibody to CXCR4. Transendothelial migration of CB CAFCs and LTC-ICs was higher than that of PB. We expanded CD34+ cells from CB in serum-free medium with thrombopoietin, flk-2 ligand, and c-kit ligand, with or without IL-3 and found that CAFCs cultured in the absence of IL-3 had a chemotactic response equivalent to noncultured cells, even after 5 weeks. However, addition of IL-3 to the culture reduced this response by 20-50%. These data indicate that SDF-1 induces chemotaxis of primitive hematopoietic cells signaling through CXCR4 and that the chemoattraction could be downmodulated by culture ex vivo. |
| Keywords: | signal transduction; unclassified drug; nonhuman; animal cell; animals; mice; stem cell factor; cell protein; cell line; membrane proteins; blood; chemotaxis; cell culture; endothelium, vascular; hematopoietic cell; hematopoietic stem cells; cell migration; cell movement; umbilical cord blood; stroma cell; antigens, cd34; thrombopoietin; stromal cell derived factor 1; receptors, cxcr4; chemokine receptor cxcr4; interleukin 3; chemokines, cxc; priority journal; article |
| Journal Title: | Journal of Clinical Investigation |
| Volume: | 105 |
| Issue: | 1 |
| ISSN: | 0021-9738 |
| Publisher: | American Society for Clinical Investigation |
| Date Published: | 2000-01-01 |
| Start Page: | 101 |
| End Page: | 111 |
| Language: | English |
| PUBMED: | 10619866 |
| PROVIDER: | scopus |
| PMCID: | PMC382585 |
| DOI: | 10.1172/JCI7954 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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