Identification of a region of Escherichia coli DnaB required for functional interaction with DnaG at the replication fork Journal Article
| Authors: | Chang, P.; Marians, K. J. |
| Article Title: | Identification of a region of Escherichia coli DnaB required for functional interaction with DnaG at the replication fork |
| Abstract: | The fundamental activities of the replicative primosomes of Escherichia coli are provided by DnaB, the replication fork DNA helicase, and DnaG, the Okazaki fragment primase. As we have demonstrated previously, DnaG is recruited to the replication fork via a transient protein-protein interaction with DnaB. Here, using site-directed amino acid mutagenesis, we have defined the region on DnaB required for this protein-protein interaction. Mutations in this region of DnaB affect the DnaB-DnaG interaction during both general priming-directed and φX174 complementary strand DNA synthesis, as well as at replication forks reconstituted in rolling circle DNA replication reactions. The behavior of the purified mutant DnaB proteins in the various replication systems suggests that access to the DnaG binding pocket on DnaB may be restricted at the replication fork. |
| Keywords: | nonhuman; dna replication; dna synthesis; amino acid substitution; protein protein interaction; structure-activity relationship; bacterial proteins; dna; escherichia coli; dna, viral; crystal structure; models, molecular; site directed mutagenesis; dna helicases; gene location; electrophoresis, polyacrylamide gel; dna b; dna primase; dnab helicases; bacteriophage phi x 174; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 275 |
| Issue: | 34 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2000-08-25 |
| Start Page: | 26187 |
| End Page: | 26195 |
| Language: | English |
| DOI: | 10.1074/jbc.M001800200 |
| PUBMED: | 10833513 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
