| Abstract: |
Heat shock protein (HSP)-peptide complexes isolated from murine cancers elicit protective immunity and T lymphocytes specific for the cancer from which the HSPs are isolated. A pilot study was designed to test the feasibility, immunogenicity and toxicity of such treatment in cancer patients. Sixteen patients with assorted advanced malignancies, which had become refractory to established therapies, were recruited. The gp96 vaccine was prepared for each patient from tumor obtained from that patient. Anti-tumor immune responses were evaluated using Elispot assays of T cells in peripheral blood after minimal in vitro stimulation. No unacceptable vaccine-related toxicities or auto-immune reactions were observed. Immunization with autologous gp96 elicited MHC I-restricted, tumor-specific CD8+ T lymphocytes in 6/12 patients immunized. In addition, expansion of the NK cell population was seen in 8/13 of patients immunized. These observations are entirely consistent with the murine experience and form a firm basis for future trials with clinical end points, using autologous, patient-specific HSP-peptide vaccines. (C) 2000 Wiley-Liss, Inc. |
| Keywords: |
adult; clinical article; treatment outcome; aged; disease-free survival; cancer patient; neoplasms; cd8 antigen; t lymphocyte; cd8-positive t-lymphocytes; antineoplastic activity; immune response; antigens, neoplasm; cancer vaccine; cancer vaccines; pilot projects; disease progression; immunogenicity; histocompatibility antigens class i; natural killer cell; killer cells, natural; autoimmunity; major histocompatibility antigen class 1; immunization; middle age; heat-shock proteins; heat shock protein; human; male; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
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