Comparison of a multiplex reverse transcriptase-polymerase chain reaction for BCR-ABL to fluorescence in situ hybridization, Southern blotting, and conventional cytogenetics in the monitoring of patients with Ph1-positive leukemias Journal Article
| Authors: | Colleoni, G. W. B.; Jhanwar, S. C.; Ladanyi, M.; Chen, B. |
| Article Title: | Comparison of a multiplex reverse transcriptase-polymerase chain reaction for BCR-ABL to fluorescence in situ hybridization, Southern blotting, and conventional cytogenetics in the monitoring of patients with Ph1-positive leukemias |
| Abstract: | A multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) assay for both major forms of BCR-ABL was compared with fluorescence in situ hybridization (FISH), karyotyping, and Southern blotting for disease monitoring in 37 follow-up bone marrow samples from 32 patients with Ph1-positive leukemia. Of these 37 samples, 33 were from patients with chronic myeloid leukemia (CML) (26 post allogeneic bone marrow transplantation [AlloBMT] and seven during interferon-α therapy) and 4 from Ph1-positive acute lymphoblastic leukemia (ALL) patients (1 post AlloBMT and 3 post high dose chemotherapy). For the 27 samples studied after AlloBMT (26 CML and 1 Ph1-positive ALL) the time after transplantation ranged from 1 to 107 months (median 47.5 months). In 8 (22%) of the 37 samples there were discrepant results among methods. The discrepancy rates relative to other techniques were: karyotyping 17% (5 of 29), Southern blotting 18% (6 of 33), multiplex RT-PCR 8% (3 of 37), and FISH 8% (3 of 37). Therefore, the relative accuracy of each method for disease monitoring in Ph1-positive leukemia was: 83% (24 of 29) for karyotyping, 82% (27 of 33) for Southern blotting, 92% (34 of 37) for FISH, and 92% (34 of 37) for multiplex RT-PCR. This multiplex RT-PCR assay appears equivalent to FISH in terms of accuracy, simplicity, and turnaround time and both are superior to Southern blot and conventional cytogenetics in the laboratory monitoring of Ph1-positive leukemias. |
| Keywords: | adolescent; adult; child; clinical article; human tissue; school child; middle aged; leukemia; human cell; alpha interferon; polymerase chain reaction; accuracy; in situ hybridization, fluorescence; reverse transcription polymerase chain reaction; cytogenetics; patient monitoring; tumor cells, cultured; chronic myeloid leukemia; acute lymphoblastic leukemia; fluorescence in situ hybridization; reverse transcriptase polymerase chain reaction; intermethod comparison; bone marrow cell; bcr abl protein; bone marrow transplantation; karyotyping; cytogenetic analysis; philadelphia 1 chromosome; southern blotting; k562 cells; philadelphia chromosome; genes, abl; leukemia, myeloid, philadelphia-positive; blotting, southern; humans; human; male; female; priority journal; article; reverse transcriptase; bcr-abl rearrangement |
| Journal Title: | Diagnostic Molecular Pathology |
| Volume: | 9 |
| Issue: | 4 |
| ISSN: | 1052-9551 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 2000-12-01 |
| Start Page: | 203 |
| End Page: | 209 |
| Language: | English |
| DOI: | 10.1097/00019606-200012000-00005 |
| PUBMED: | 11129444 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work