Association of the bloom syndrome protein with topoisomerase IIIα in somatic and meiotic cells Journal Article
| Authors: | Johnson, F. B.; Lombard, D. B.; Neff, N. F.; Mastrangelo, M. A.; Dewolf, W.; Ellis, N. A.; Marciniak, R. A.; Yin, Y.; Jaenisch, R.; Guarente, L. |
| Article Title: | Association of the bloom syndrome protein with topoisomerase IIIα in somatic and meiotic cells |
| Abstract: | Bloom syndrome (BS) is characterized by genomic instability and cancer susceptibility caused by defects in BLM, a DNA helicase of the RecQ-family (J. German and N.A. Ellis, The Genetic Basis of Human Cancer, pp. 301-316, 1998). RecQ helicases and topoisomerase III proteins interact physically and functionally in yeast (S. Gangloff et al., Mol. Cell. Biol., 14: 8391-8398, 1994) and in Escherichia coli can function together to enable passage of double-stranded DNA (F. G. Harmon et al., Mol. Cell, 3: 611-620, 1999). We demonstrate in somatic and meiotic human cells an association between BLM and topoisomerase IIIα. These proteins colocalize in promyelocytic leukemia protein nuclear bodies, and this localization is disrupted in BS cells. Thus, mechanisms by which RecQ helicases and topoisomerase III proteins cooperate to maintain genomic stability in model organisms likely apply to humans. |
| Keywords: | human cell; nonhuman; protein localization; meiosis; somatic cell; cancer susceptibility; tumor cells, cultured; enzyme activity; gene expression regulation; cellular distribution; helicase; adenosine triphosphatases; dna helicases; dna topoisomerase; dna topoisomerases, type i; bloom syndrome; humans; human; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 60 |
| Issue: | 5 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2000-03-01 |
| Start Page: | 1162 |
| End Page: | 1167 |
| Language: | English |
| PUBMED: | 10728666 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
