Adenoviral vector-mediated gene transfer to primitive human hematopoietic progenitor cells: Assessment of transduction and toxicity in long-term culture Journal Article
| Authors: | Mackenzie, K. L.; Hackett, N. R.; Crystal, R. G.; Moore, M. A. S. |
| Article Title: | Adenoviral vector-mediated gene transfer to primitive human hematopoietic progenitor cells: Assessment of transduction and toxicity in long-term culture |
| Abstract: | Adenoviral gene transfer to primitive hematopoietic progenitor cells (HPCs) would be useful in gene therapy applications where transient, high- level transgene expression is required. In the present investigations, we have used an adenoviral vector expressing the green fluorescent protein (AdGFP) to quantity transduction of primitive HPCs and assess adenoviral- associated toxicity in long-term culture. Here we show that a cytokine cocktail protects mass populations of CD34+ cells and primary colony forming unit-cultures (CFU-Cs) from toxicity, enabling transduction of up to 79% of CD34+ cells. Transduction of CFU-Cs and more primitive HPCs was quantified following fluorescence activated cell sorting for green flourescence protein expression. Our results demonstrate transduction of 45% of primary CFU-Cs, 33% of week-5 cobblestone area forming cells (CAFCs), and 18% of week-5 CFU- Cs. However, AdGFP infection inhibited proliferation of more primitive cells. Although there was no apparent quantitative change in week-5 CAFCs, the clonogenic capacity of week-5 AdGFP-infected cells was reduced by 40% (P < .01) when compared with mock-infected cells. Adenoviral toxicity specifically affected the transduced subset of primitive HPCs. Transduction of primitive cells is therefore probably underestimated by week-5 CFU-Cs and more accurately indicated by week-5 CAFCs. These studies provide the first functional and quantitative evidence of adenoviral transduction of primitive HPCs. However, further investigations will be necessary to overcome adenoviral toxicity toward primitive HPCs before adenoviral vectors can be considered a safe option for gene therapy. (C) 2000 by The American Society of Hematology. |
| Keywords: | signal transduction; controlled study; human tissue; human cell; histopathology; drug safety; cell proliferation; cells, cultured; cd34 antigen; green fluorescent protein; transfection; time factors; gene transfer; genetic vectors; luminescent proteins; cell culture; membrane glycoproteins; gene therapy; hematopoietic stem cells; green fluorescent proteins; hematopoietic stem cell; antigens, cd; colony-forming units assay; antigens, cd34; hematopoietic stem cell mobilization; antigens, cd38; antigens, differentiation; adenoviridae; virus vector; gene transfer techniques; humans; human; priority journal; article; colony forming unit c; adp-ribosyl cyclase; nad+ nucleosidase |
| Journal Title: | Blood |
| Volume: | 96 |
| Issue: | 1 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 2000-07-01 |
| Start Page: | 100 |
| End Page: | 108 |
| Language: | English |
| PUBMED: | 10891437 |
| PROVIDER: | scopus |
| DOI: | 10.1182/blood.V96.1.100 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work