MUC-6 mucin is a major component of 'blood group substance' from human ovarian cyst fluid Journal Article
| Authors: | Lloyd, K. O.; Yin, B. W. T.; Tempst, P.; Erdjument-Bromage, H. |
| Article Title: | MUC-6 mucin is a major component of 'blood group substance' from human ovarian cyst fluid |
| Abstract: | Ovarian cyst fluid has been a valuable source of the mucins (traditionally termed 'blood group substances') that were used for the elucidation of the structures of the ABO Lewis blood group determinants, but the identity of the mucin peptide core(s) carrying these carbohydrate specificities is not known. An ovarian cyst fluid mucin was purified, deglycosylated with HF and digested with trypsin or chymotrypsin to yield a number of peptides. Amino acid sequencing of these peptides yielded five different sequences which showed complete or partial homology to the MUC-6 apomucin deduced from DNA sequencing. As no other sequences were identified, it is concluded that MUC-6 is the major mucin core structure of ovarian cyst fluid mucin. Copyright (C) 2000 Elsevier Science B.V. |
| Keywords: | ovarian cancer; cyst fluid; peptide; adenoma; amino acid sequence; molecular sequence data; protein purification; epitope; glycosylation; amino acids; protein structure; chymotrypsin; carbohydrate; polyacrylamide gel electrophoresis; ovary cyst; trypsin; mucin; mucins; amino acid composition; ovarian cysts; blood group; humans; human; female; priority journal; article; deglycosylation; blood group antigen; muc-6 |
| Journal Title: | Biochimica et Biophysica Acta (BBA) - General Subjects |
| Volume: | 1474 |
| Issue: | 3 |
| ISSN: | 0304-4165 |
| Publisher: | Elsevier B.V. |
| Date Published: | 2000-05-01 |
| Start Page: | 410 |
| End Page: | 414 |
| Language: | English |
| DOI: | 10.1016/s0304-4165(00)00037-4 |
| PUBMED: | 10779694 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work