Cytokeratin immunoreactivity in Ewing's sarcoma: Prevalence in 50 cases confirmed by molecular diagnostic studies Journal Article
| Authors: | Gu, M.; Antonescu, C. R.; Guiter, G.; Huvos, A. G.; Ladanyi, M.; Zakowski, M. F. |
| Article Title: | Cytokeratin immunoreactivity in Ewing's sarcoma: Prevalence in 50 cases confirmed by molecular diagnostic studies |
| Abstract: | Ewing's sarcoma (ES) and primitive neuroectodermal tumor (PNET) are characterized by the presence of the specific t(11;22)(q24;q12) or variants thereof, producing diagnostic EWS fusion transcripts. Cytokeratin has been reported sporadically to be expressed in some cases of ES/PNET. However, its prevalence has not been assessed systematically in a series of cases with confirmatory molecular or cytogenetic evidence of a diagnostic translocation. We present in detail three index patients in whom strong cytokeratin immunoreactivity was a confounding factor in the diagnosis. To establish further the prevalence of cytokeratin immunoreactivity in a series of well- characterized ES/PNET, we then performed immunohistochemical studies with antibodies CAM5.2 and AE1/AE3 on 50 cases of ES/PNET diagnosed at Memorial Sloan-Kettering Cancer Center in which molecular evidence of a specific ES/PNET-associated translocation were available. Immunoreactivity to cytokeratin was present in 10 cases (20%), in five diffusely and five focally. There was no significant association between cytokeratin expression and the following parameters: patient age, sex, skeletal and extraskeletal primary site, and the type of EWS fusion transcript. Cytokeratin expression, a manifestation of epithelial differentiation, is present in as many as 20% of ES/PNET in either a diffuse or focal pattern. |
| Keywords: | immunohistochemistry; adult; child; school child; aged; case report; prevalence; monoclonal antibody; ewing sarcoma; gene fusion; molecular biology; cytokeratin; keratins; sarcoma, ewing's; neoplasms, multiple primary; neuroectoderm tumor; neuroectodermal tumors, primitive; primitive neuroectodermal tumor; chromosome 22; ewing's sarcoma; chromosome translocation 11; humans; human; male; female; article |
| Journal Title: | American Journal of Surgical Pathology |
| Volume: | 24 |
| Issue: | 3 |
| ISSN: | 0147-5185 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 2000-03-01 |
| Start Page: | 410 |
| End Page: | 416 |
| Language: | English |
| DOI: | 10.1097/00000478-200003000-00010 |
| PUBMED: | 10716155 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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