Innate immune defenses mediated by two ilc subsets are critical for protection against acute clostridium difficile infection Journal Article
| Authors: | Abt, M. C.; Lewis, B. B.; Caballero, S.; Xiong, H.; Carter, R. A.; Susac, B.; Ling, L.; Leiner, I.; Pamer, E. G. |
| Article Title: | Innate immune defenses mediated by two ilc subsets are critical for protection against acute clostridium difficile infection |
| Abstract: | Infection with the opportunistic enteric pathogen Clostridium difficile is an increasingly common clinical complication that follows antibiotic treatment- induced gut microbiota perturbation. Innate lymphoid cells (ILCs) are early responders to enteric pathogens; however, their role during C. difficile infection is undefined. To identify immune pathways that mediate recovery from C. difficile infection, we challenged C57BL/6, Rag1-/- (which lack T and B cells), and Rag2-/- Il2rg-/- (Raggc-/-) mice (which additionally lack ILCs) with C. difficile. In contrast to Rag1-/- mice, ILC-deficient Raggc-/- mice rapidly succumbed to infection. Rag1-/- but not Raggc-/- mice upregulate expression of ILC1- or ILC3-associated proteins following C. difficile infection. Protection against infection was restored by transferring ILCs into Raggc-/- mice. While ILC3s made a minor contribution to resistance, loss of IFN-g or T-betexpressing ILC1s in Rag1-/- mice increased susceptibility to C. difficile. These data demonstrate a critical role for ILC1s in defense against C. difficile. © 2015 Elsevier Inc. |
| Keywords: | controlled study; protein expression; unclassified drug; nonhuman; animal cell; mouse; animal tissue; protein depletion; animal experiment; animal model; protein; gamma interferon; adoptive transfer; innate immunity; upregulation; clostridium difficile infection; transcription factor t bet; host resistance; infection sensitivity; interleukin 22; lymphoid cell; infection resistance; priority journal; article; ilc1 associated protein; ilc3 associated protein |
| Journal Title: | Cell Host & Microbe |
| Volume: | 18 |
| Issue: | 1 |
| ISSN: | 1931-3128 |
| Publisher: | Cell Press |
| Date Published: | 2015-07-08 |
| Start Page: | 27 |
| End Page: | 37 |
| Language: | English |
| DOI: | 10.1016/j.chom.2015.06.011 |
| PROVIDER: | scopus |
| PMCID: | PMC4537644 |
| PUBMED: | 26159718 |
| DOI/URL: | |
| Notes: | Export Date: 2 November 2015 -- Source: Scopus |
