Metastatic competence can emerge with selection of preexisting oncogenic alleles without a need of new mutations Journal Article
| Authors: | Jacob, L. S.; Vanharanta, S.; Obenauf, A. C.; Pirun, M.; Viale, A.; Socci, N. D.; Massagué, J. |
| Article Title: | Metastatic competence can emerge with selection of preexisting oncogenic alleles without a need of new mutations |
| Abstract: | Several experimental models faithfully recapitulate many important facets of human metastatic disease. Here, we have performed whole-exome sequencing in five widely used experimental metastasis models that were independently derived through in vivo selection from heterogeneous human cancer cell lines. In addition to providing an important characterization of these model systems, our study examines the genetic evolution of metastatic phenotypes. We found that in vivo selected highly metastatic cell populations showed little genetic divergence from the corresponding parental population. However, selection of genetic variations that preexisted in parental populations, including the well-established oncogenic mutations KRASG13D and BRAFG464V, was associated with increased metastatic capability. Conversely, expression of the wild-type BRAF allele in metastatic cells inhibited metastatic outgrowth as well as tumor initiation in mice. Our findings establish that metastatic competence can arise from heterogeneous cancer cell populations without the need for acquisition of additional mutations and that such competence can benefit from further selection of tumor-initiating mutations that seed primary tumorigenesis. Cancer Res; 75(18); 3713-9. © 2015 American Association for Cancer Research. |
| Keywords: | controlled study; gene mutation; gene sequence; exon; missense mutation; cancer growth; nonhuman; polymerase chain reaction; mouse; phenotype; animal tissue; tumor volume; animal experiment; genetic variability; cell population; carcinogenesis; cancer inhibition; oncogene; kidney adenoma; cancer cell; pleura effusion; base pairing; metastasis potential; oncogene k ras; b raf kinase; genetic conservation; bioluminescence; von hippel lindau protein; genetic selection; phylogeny; copy number variation; triple negative breast cancer; exome; high throughput sequencing; female; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 75 |
| Issue: | 18 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2015-09-15 |
| Start Page: | 3713 |
| End Page: | 3719 |
| Language: | English |
| DOI: | 10.1158/0008-5472.can-15-0562 |
| PROVIDER: | scopus |
| PMCID: | PMC4573898 |
| PUBMED: | 26208905 |
| DOI/URL: | |
| Notes: | Export Date: 2 November 2015 -- Source: Scopus |
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