Local and sex-specific biases in crossover vs. noncrossover outcomes at meiotic recombination hot spots in mice Journal Article

   

Authors:	De Boer, E.; Jasin, M.; Keeney, S.
Article Title:	Local and sex-specific biases in crossover vs. noncrossover outcomes at meiotic recombination hot spots in mice
Abstract:	Meiotic recombination initiated by programmed double-strand breaks (DSBs) yields two types of interhomolog recombination products, crossovers and noncrossovers, but what determines whether a DSB will yield a crossover or noncrossover is not understood. In this study, we analyzed the influence of sex and chromosomal location on mammalian recombination outcomes by constructing fine-scale recombination maps in both males and females at two mouse hot spots located in different regions of the same chromosome. These include the most comprehensive maps of recombination hot spots in oocytes to date. One hot spot, located centrally on chromosome 1, behaved similarly in male and female meiosis: Crossovers and noncrossovers formed at comparable levels and ratios in both sexes. In contrast, at a distal hot spot, crossovers were recovered only in males even though noncrossovers were obtained at similar frequencies in both sexes. These findings reveal an example of extreme sex-specific bias in recombination outcome. We further found that estimates of relative DSB levels are surprisingly poor predictors of relative crossover frequencies between hot spots in males. Our results demonstrate that the outcome of mammalian meiotic recombination can be biased, that this bias can vary depending on location and cellular context, and that DSB frequency is not the only determinant of crossover frequency. © 2015 de Boer et al.
Keywords:	controlled study; nonhuman; chromosome 1; mouse; meiosis; mammalia; mus; haplotype; gene conversion; genetic recombination; double stranded dna break; genome; sex difference; recombination; crossing over; meiotic recombination; frequency; spo11; recombinant dna; male; female; priority journal; article; hot spot; recombinant inbred strain
Journal Title:	Genes and Development
Volume:	29
Issue:	16
ISSN:	0890-9369
Publisher:	Cold Spring Harbor Laboratory Press
Date Published:	2015-08-15
Start Page:	1721
End Page:	1733
Language:	English
DOI:	10.1101/gad.265561.115
PROVIDER:	scopus
PMCID:	PMC4561481
PUBMED:	26251527
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84940035439&partnerID=40&md5=9ff3019f0f594d410bcea361cf9760ea
Notes:	Export Date: 2 October 2015 -- Source: Scopus

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