Label-free enrichment of cancer cells using negative selection (depletion) of wbcs with elastomeric negative acoustic contrast particles and acoustophoresis Conference Paper

  


Authors: Cushing, K. W.; Undvall, E.; Haflidadottir, B.; Lenshof, A.; Magnusson, C.; Lilja, H.; Laurell, T.
Title: Label-free enrichment of cancer cells using negative selection (depletion) of wbcs with elastomeric negative acoustic contrast particles and acoustophoresis
Conference Title: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abstract: In an effort to bioengineer elastomeric particles (EPs), with negative acoustic contrast factor, to capture cells, we have successfully functionalized EPs with anti-human CD45 antibodies that enable capturing of WBCs from human RBC lysed blood samples. We demonstrate that the negative selection of WBCs with EPs and acoustophoresis can be employed to reduce WBC contamination while enriching label-free prostate cancer cells (DU145) within the central fraction of our acoustophoresis chip. © 14CBMS.
Keywords: circulating tumor cells; diseases; acoustophoresis; functionalized; negative selection; label free; prostate cancer cells; elastomeric particles; label-free enrichment; capture cells; contrast factor
Journal Title Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Conference Dates: 2014 Oct 26-30
Conference Location: San Antonio, TX
ISBN: 978-0-9798064-7-6
Publisher: Chemical and Biological Microsystems Society  
Date Published: 2014-01-01
Start Page: 2540
End Page: 2541
Language: English
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84941712257&partnerID=40&md5=c81eabe7feb742284e2906c48efeb973
Notes: Int. Conf. Miniaturized Syst. Chem. Life Sci., MicroTAS -- Conference code: 113844 -- Export Date: 2 October 2015 -- Analytical Chemistry; CBMS; Indiana University (IU); Institute of Microchemical Technology (IMT); LPKF Laser and Electronics -- 26 October 2014 through 30 October 2014 -- Source: Scopus