Ribosomal RNA gene sequencing for early diagnosis of Blastomyces dermatitidis infection Journal Article
| Authors: | Morjaria, S.; Otto, C.; Moreira, A.; Chung, R.; Hatzoglou, V.; Pillai, M.; Banaei, N.; Tang, Y. W.; Figueroa, C. J. |
| Article Title: | Ribosomal RNA gene sequencing for early diagnosis of Blastomyces dermatitidis infection |
| Abstract: | Prompt detection and identification of fungal pathogens at the genus and species level is critical in order to provide timely antifungal therapy. Here, we highlight the vital role of molecular diagnostics in achieving a fast and definitive diagnosis of disseminated blastomycosis in a diabetic patient presenting as a brain mass initially thought to be tumoral in nature. A broad-range PCR amplification and sequencing of the fungal ribosomal RNA genes on brain biopsy tissue obtained during elective craniotomy revealed a final microbial identification of Ajellomyces dermatitidis (telemorph of Blastomyces dermatitidis). © 2015 The Authors. |
| Keywords: | human tissue; treatment outcome; aged; histopathology; case report; neuroimaging; nuclear magnetic resonance imaging; polymerase chain reaction; gene amplification; steroid; emergency ward; disease severity; neutrophil; tonic clonic seizure; early diagnosis; intraoperative period; craniotomy; physical examination; ribosome rna; anticonvulsive agent; antibody detection; brain biopsy; brain necrosis; brain edema; rna sequence; antimicrobial therapy; arm weakness; elective surgery; repeat procedure; molecular diagnosis; anticonvulsant therapy; granulomatous inflammation; brain tissue; amphotericin b lipid complex; corticosteroid therapy; systemic mycosis; diabetic patient; blastomyces dermatitidis; fungal detection; blastomycosis; brain abscess; fungal rna; ajellomyces dermatitidis; fungus antibody; human; male; article; suppuration; fungal diagnostics; fungal rna sequencing; internal transcribed spacer 2; frontoparietal cortex |
| Journal Title: | International Journal of Infectious Diseases |
| Volume: | 37 |
| ISSN: | 1201-9712 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2015-08-01 |
| Start Page: | 122 |
| End Page: | 124 |
| Language: | English |
| DOI: | 10.1016/j.ijid.2015.06.017 |
| PROVIDER: | scopus |
| PUBMED: | 26129971 |
| DOI/URL: | |
| Notes: | Export Date: 2 September 2015 -- Source: Scopus |
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