Targeting DOT1L and HOX gene expression in MLL-rearranged leukemia and beyond Journal Article
| Authors: | Chen, C. W.; Armstrong, S. A. |
| Article Title: | Targeting DOT1L and HOX gene expression in MLL-rearranged leukemia and beyond |
| Abstract: | Leukemias harboring mixed-lineage leukemia gene (MLL1) abnormalities are associated with poor clinical outcomes, and new therapeutic approaches are desperately needed. Rearrangement of the MLL1 gene generates chimeric proteins that fuse the NH<inf>3</inf> terminus of MLL1 to the COOH terminus of its translocation partners. These MLL1 fusion oncoproteins drive the expression of homeobox genes such as HOXA cluster genes and myeloid ecotropic viral integration site 1 homolog (MEIS1), which are known to induce leukemic transformation of hematopoietic progenitors. Genomewide histone methylation studies have revealed that the abnormal expression of MLL1 fusion target genes is associated with high levels of H3K79 methylation at these gene loci. The only known enzyme that catalyzes methylation of H3K79 is disruptor of telomeric-silencing 1-like (DOT1L). Loss-of-function mouse models, as well as small molecular inhibitors of DOT1L, illustrate that leukemias driven by MLL1 translocations are dependent on DOT1L enzymatic activity for proliferation and for the maintenance of HOXA gene expression. Furthermore, DOT1L also appears to be important for HOXA gene expression in other settings including leukemias with select genetic abnormalities. These discoveries have established a foundation for disease-specific therapies that target chromatin modifications in highly malignant leukemias harboring specific genetic abnormalities. This review focuses on the molecular mechanisms underlying MLL1 translocation-driven leukemogenesis and the latest progress on DOT1L-targeted epigenetic therapies for MLL1-rearranged and other leukemias. © 2015 ISEH - International Society for Experimental Hematology. |
| Keywords: | leukemia; gene translocation; review; nonhuman; gene; gene targeting; transcription initiation; gene function; gene expression regulation; gene rearrangement; transcription regulation; epigenetics; gene interaction; leukemogenesis; chromatin assembly and disassembly; gene structure; transcription elongation; histone methylation; hox gene; mll1 gene; human; priority journal; dot1l gene |
| Journal Title: | Experimental Hematology |
| Volume: | 43 |
| Issue: | 8 |
| ISSN: | 0301-472X |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2015-08-01 |
| Start Page: | 673 |
| End Page: | 684 |
| Language: | English |
| DOI: | 10.1016/j.exphem.2015.05.012 |
| PROVIDER: | scopus |
| PMCID: | PMC4540610 |
| PUBMED: | 26118503 |
| DOI/URL: | |
| Notes: | Export Date: 2 September 2015 -- Source: Scopus |
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