Gamma-secretase inhibitors and modulators induce distinct conformational changes in the active sites of gamma-secretase and signal peptide peptidase Journal Article
| Authors: | Gertsik, N.; Chau, D. M.; Li, Y. M. |
| Article Title: | Gamma-secretase inhibitors and modulators induce distinct conformational changes in the active sites of gamma-secretase and signal peptide peptidase |
| Abstract: | γ-Secretase inhibitors (GSIs) and modulators (GSMs) are at the frontline of cancer and Alzheimers disease research, respectively. While both are therapeutically promising, not much is known about their interactions with proteins other than γ-secretase. Signal peptide peptidase (SPP), like γ-secretase, is a multispan transmembrane aspartyl protease that catalyzes regulated intramembrane proteolysis. We used active site-directed photophore walking probes to study the effects of different GSIs and GSMs on the active sites of γ-secretase and SPP and found that nontransition state GSIs inhibit labeling of γ-secretase by activity-based probes but enhance labeling of SPP. The opposite is true of GSMs, which have little effect on the labeling of γ-secretase but diminish labeling of SPP. These results demonstrate that GSIs and GSMs are altering the structure of not only γ-secretase but also SPP, leading to potential changes in enzyme activity and specificity that may impact the clinical outcomes of these molecules. © 2015 American Chemical Society. |
| Journal Title: | ACS Chemical Biology |
| Volume: | 10 |
| Issue: | 8 |
| ISSN: | 1554-8929 |
| Publisher: | American Chemical Society |
| Date Published: | 2015-08-01 |
| Start Page: | 1925 |
| End Page: | 1931 |
| Language: | English |
| DOI: | 10.1021/acschembio.5b00321 |
| PROVIDER: | scopus |
| PUBMED: | 26030233 |
| PMCID: | PMC5436900 |
| DOI/URL: | |
| Notes: | Export Date: 2 September 2015 -- Source: Scopus |
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