A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs Journal Article


Authors: Fulga, T. A.; McNeill, E. M.; Binari, R.; Yelick, J.; Blanche, A.; Booker, M.; Steinkraus, B. R.; Schnall-Levin, M.; Zhao, Y.; DeLuca, T.; Bejarano, F. ; Han, Z.; Lai, E. C.; Wall, D. P.; Perrimon, N.; Van Vactor, D.
Article Title: A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs
Abstract: Although the impact of microRNAs (miRNAs) in development and disease is well established, understanding the function of individual miRNAs remains challenging. Development of competitive inhibitor molecules such as miRNA sponges has allowed the community to address individual miRNA function in vivo. However, the application of these loss-of-function strategies has been limited. Here we offer a comprehensive library of 141 conditional miRNA sponges targeting well-conserved miRNAs in Drosophila. Ubiquitous miRNA sponge delivery and consequent systemic miRNA inhibition uncovers a relatively small number of miRNA families underlying viability and gross morphogenesis, with false discovery rates in the 4-8% range. In contrast, tissue-specific silencing of muscle-enriched miRNAs reveals a surprisingly large number of novel miRNA contributions to the maintenance of adult indirect flight muscle structure and function. A strong correlation between miRNA abundance and physiological relevance is not observed, underscoring the importance of unbiased screens when assessing the contributions of miRNAs to complex biological processes.
Keywords: animal; micrornas; zebrafish; neurodegeneration; expression; messenger-rna; wing development; melanogaster; translational repression; muscle differentiation; bantam mirna
Journal Title: Nature Communications
Volume: 6
ISSN: 2041-1723
Publisher: Nature Publishing Group  
Date Published: 2015-06-17
Start Page: 7279
Language: English
ACCESSION: WOS:000357170800006
DOI: 10.1038/ncomms8279
PROVIDER: wos
PMCID: PMC4471878
PUBMED: 26081261
Notes: Article -- 7279 -- Source: Wos
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  1. Eric C Lai
    159 Lai