Active disruption of an RNA-protein interaction by a DExH/D RNA helicase Journal Article
| Authors: | Jankowsky, E.; Gross, C. H.; Shuman, S.; Pyle, A. M. |
| Article Title: | Active disruption of an RNA-protein interaction by a DExH/D RNA helicase |
| Abstract: | All aspects of cellular RNA metabolism and the replication of many viruses require DExH/D proteins that manipulate RNA in a manner that requires nucleoside triphosphates. Although DExH/D proteins have been shown to unwind purified RNA duplexes, most RNA molecules in the cellular environment are complexed with proteins. It has therefore been speculated that DExH/D proteins may also affect RNA-protein interactions. We demonstrate that the DExH protein NPH-II from vaccinia virus can displace the protein U1A from RNA in an active adenosine triphosphate-dependent fashion. NPH-II increases the rate of U1A dissociation by more than three orders of magnitude while retaining helicase processivity. This indicates that DExH/D proteins can effectively catalyze protein displacement from RNA and thereby participate in the structural reorganization of ribonucleoprotein assemblies. |
| Keywords: | spliceosome; atp hydrolysis; specificity; binding; messenger-rna; box; u1a protein |
| Journal Title: | Science |
| Volume: | 291 |
| Issue: | 5501 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 2001-01-05 |
| Start Page: | 121 |
| End Page: | 125 |
| Language: | English |
| ACCESSION: | WOS:000166259100048 |
| DOI: | 10.1126/science.291.5501.121 |
| PROVIDER: | wos |
| PUBMED: | 11141562 |
| Notes: | Article -- Source: Wos |
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