A small molecule designed to bind to the adenine nucleotide pocket of Hsp90 causes Her2 degradation and the growth arrest and differentiation of breast cancer cells Journal Article

  

Authors:	Chiosis, G.; Timaul, M. N.; Lucas, B.; Munster, P. N.; Zheng, F. F.; Sepp-Lorenzino, L.; Rosen, N.
Article Title:	A small molecule designed to bind to the adenine nucleotide pocket of Hsp90 causes Her2 degradation and the growth arrest and differentiation of breast cancer cells
Abstract:	Background: The Hsp90s contain a conserved pocket that binds ATP/ADP and plays an important role in the regulation of chaperone function. Occupancy of this pocket by several natural products (geldanamycin (GM) and radicicol) alters Hsp90 function and results in the degradation of a subset of proteins (i.e. steroid receptors, Her2, Raf). We have used the structural features of this pocket to design a small molecule inhibitor of Hsp90. Results: The designed small molecule PU3 competes with GM for Hsp90 binding with a relative affinity of 15-20 muM. PU3 induces degradation of proteins, including Her2, in a manner similar to GM. Furthermore, PU3 inhibits the growth of breast cancer cells causing retinoblastoma protein hypophosphorylation, G1 arrest and differentiation. Conclusions: PU3 is representative of a novel class of synthetic compounds that binds to Hsp90 and inhibits the proliferation of cancer cells. These reagents could provide a new strategy for the treatment of cancers. (C) 2001 Elsevier Science Ltd. All rights reserved.
Keywords:	proteins; her2; geldanamycin; chaperone; inhibition; complex; target; ansamycins; agent; hsp90 inhibitor; novel anti-tumor agent
Journal Title:	Chemistry and Biology
Volume:	8
Issue:	3
ISSN:	1074-5521
Publisher:	Elsevier Inc.
Date Published:	2001-03-01
Start Page:	289
End Page:	299
Language:	English
ACCESSION:	WOS:000168383900007
DOI:	10.1016/s1074-5521(01)00015-1
PROVIDER:	wos
PUBMED:	11306353
Notes:	Article -- Source: Wos

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