Uptake of (45)Ca by mitochondria of Trigonella foenum-graecum as influenced by selenium and mimosine - Detailed kinetic analyses Journal Article
| Authors: | Sreekala, M.; Lalitha, K. |
| Article Title: | Uptake of (45)Ca by mitochondria of Trigonella foenum-graecum as influenced by selenium and mimosine - Detailed kinetic analyses |
| Abstract: | Mitochondria from Trigonella foenum-graecum seedlings grown independently in the presence of either selenium (0.75 ppm) or mimosine (0.1 mM) exhibited respiration-stimulated energy-dependent uptake of Ca2+. Uptake studies were carried out independently at a series of Ca2+ concentrations at two different levels: (1) 1-20 muM and (2) 25-1500 muM. Levels of uptake were 50-100% higher in the mitochondria of seedlings of both the Se and mimosine groups. Detailed kinetic analyses revealed negative cooperative effects operative during uptake of Ca2+ at 25-1500 muM given in the medium. Hill coefficients for Ca2+ uptake by the mitochondria of different groups remained unchanged (nH, 0.75). Biphasic Scatchard plots were concave upward, suggestive of two classes of binding sites. High-affinity binding sites were estimated to be 16 nmol/mg protein with dissociation constant (K-Ca) of 2.5 x 10(9) L/mol. In contrast, graphical analyses of the uptake of Ca2+ in the range 1-20 muM in the medium revealed cooperative effects of positive nature. The present study demonstrates mixed cooperative effects during Ca2+ uptake by mitochondria from seedlings of T. foenum-graecum. |
| Keywords: | metabolism; stress; oxidative; deficiency; selenium : plant mitochondria; ca uptake; kinetics of ca-45 uptake; trigonella foenum-graecum : essentiality of se; ca-45 uptake; effect of; mimosine; mimosine : trigonella foenum-graecum; effect on; plant mitochondria; plant mitochondria : effect of mimosine; se; rat-liver mitochondria; calcium-transport; corn mitochondria; mitigation; seedlings |
| Journal Title: | Biological Trace Element Research |
| Volume: | 82 |
| Issue: | 1-3 |
| ISSN: | 0163-4984 |
| Publisher: | Springer |
| Date Published: | 2001-06-01 |
| Start Page: | 217 |
| End Page: | 229 |
| Language: | English |
| ACCESSION: | WOS:000171653500019 |
| DOI: | 10.1385/bter:82:1-3:217 |
| PROVIDER: | wos |
| PUBMED: | 11697770 |
| Notes: | Article -- Source: Wos |
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