Non-coding RNA generated following lariat debranching mediates targeting of AID to DNA Journal Article
| Authors: | Zheng, S.; Vuong, B. Q.; Vaidyanathan, B.; Lin, J. Y.; Huang, F. T.; Chaudhuri, J. |
| Article Title: | Non-coding RNA generated following lariat debranching mediates targeting of AID to DNA |
| Abstract: | Transcription through immunoglobulin switch (S) regions is essential for class switch recombination (CSR), but no molecular function of the transcripts has been described. Likewise, recruitment of activation-induced cytidine deaminase (AID) to S regions is critical for CSR; however, the underlying mechanism has not been fully elucidated. Here, we demonstrate that intronic switch RNA acts in trans to target AID to S region DNA. AID binds directly to switch RNA through G-quadruplexes formed by the RNA molecules. Disruption of this interaction by mutation of a key residue in the putative RNA-binding domain of AID impairs recruitment of AID to S region DNA, thereby abolishing CSR. Additionally, inhibition of RNA lariat processing leads to loss of AID localization to S regions and compromises CSR; both defects can be rescued by exogenous expression of switch transcripts in a sequence-specific manner. These studies uncover an RNA-mediated mechanism of targeting AID to DNA. © 2015 Elsevier Inc. |
| Keywords: | controlled study; unclassified drug; mutation; nonhuman; animal cell; mouse; rna; activation induced cytidine deaminase; dna; untranslated rna; rna binding; guanine quadruplex; enzyme; priority journal; article; lariat debranching enzyme |
| Journal Title: | Cell |
| Volume: | 161 |
| Issue: | 4 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 2015-05-07 |
| Start Page: | 762 |
| End Page: | 773 |
| Language: | English |
| DOI: | 10.1016/j.cell.2015.03.020 |
| PROVIDER: | scopus |
| PMCID: | PMC4426339 |
| PUBMED: | 25957684 |
| DOI/URL: | |
| Notes: | Export Date: 3 June 2015 -- Source: Scopus |
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