Maintenance of TCR clonality in T cells expressing genes for two TCR heterodimers Journal Article

   


Authors: Sant'angelo, D. B.; Cresswell, P.; Janeway, C. A. Jr; Denzin, L. K.
Article Title: Maintenance of TCR clonality in T cells expressing genes for two TCR heterodimers
Abstract: T cell receptor (TCR) allelic exclusion is believed to be primarily mediated by suppression of further recombination at the TCR locus after the expression of a functional TCR protein. Genetic allelic exclusion has been shown to be leaky for the β chain and, more commonly, for the α chain. Here, we demonstrate an additional mechanism by which T cells can maintain monoclonality. T cells from double TCR transgenic mice express only one or the other of the two available TCRs at the cell surface. This "functional allelic exclusion" is apparently due to control of the TCR assembly process because these T cells express RNA and protein for all four transgenic TCR proteins. Lack of cell surface expression of the second TCR may be controlled by a failure to assemble the TCR heterodimer.
Keywords: controlled study; protein expression; nonhuman; flow cytometry; t lymphocyte; t-lymphocytes; animal cell; mouse; allele; gene expression; protein assembly; animal experiment; alleles; transgenic mouse; t lymphocyte receptor; reverse transcriptase polymerase chain reaction; rna, messenger; lymphocyte clone; rna translation; dimerization; transgenes; receptors, antigen, t-cell, alpha-beta; rna transcription; dimer; humans; priority journal; article
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 98
Issue: 12
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2001-06-05
Start Page: 6824
End Page: 6829
Language: English
DOI: 10.1073/pnas.121179998
PUBMED: 11381132
PROVIDER: scopus
PMCID: PMC34437
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0035811035&partnerID=40&md5=59ffe9190c3e7628953e29ad83e8628d
Notes: Export Date: 21 May 2015 -- Source: Scopus
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MSK Authors
  1. Lisa K Denzin
    22 Denzin
  2. Derek B Sant'angelo
    46 Sant'angelo
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