Membrane lipid rafts are necessary for the maintenance of the α7 nicotinic acetylcholine receptor in somatic spines of ciliary neurons Journal Article

Authors: Bruses, J. L.; Chauvet, N.; Rutishauser, U.
Article Title: Membrane lipid rafts are necessary for the maintenance of the α7 nicotinic acetylcholine receptor in somatic spines of ciliary neurons
Abstract: Calcium-permeable neurotransmitter receptors are concentrated into structurally and biochemically isolated cellular compartments to localize calcium-mediated events during neuro-transmission. The cytoplasmic membrane contains lipid microdomains called lipid rafts, which can gather into microscopically visible clusters, and thus the association of a particular protein with lipid rafts can result in its redistribution on the cell surface. The present study asks whether lipid rafts participate in the formation and maintenance of the calcium-permeable α7-subunit nicotinic acetylcholine receptor (α7nAChR) clusters found in somatic spines of ciliary neurons. Lipid rafts and α7nAChR become progressively colocalized within somatic spines during synaptogenesis. To determine whether these rafts are required for the maintenance of α7nAChR aggregates, cholesterol was extracted from dissociated ciliary neurons by treatment with methyl-β-cyclodextrin. This treatment caused the dispersion of lipid rafts and the redistribution of α7nAChR into small clusters over the cell surface, suggesting that the integrity of lipid rafts is required to maintain the receptor clustering. However, lipid raft dispersion also caused the depolymerization of the F-actin cytoskeleton, which can also tether the receptor at specific sites. To assess whether interaction between rafts and α7nAChR is independent of F-actin filaments, the lipid raft patches were stabilized with a combination of the cholera toxin B subunit (CTX), which specifically binds to the raft component ganglioside GM1, and an antibody against CTX. The stabilized rafts were then treated with latrunculin-A to depolymerize F-actin. Under these conditions, large patches of CTX persisted and were colocalized with α7nAChR, indicating that the aggregates of receptors can be maintained independently of the underlying F-actin cytoskeleton. Moreover, it was found that the α7nAChR is resistant to detergent extraction at 4°C and floats with the caveolin-containing lipid-rich fraction during density gradient centrifugation, properties that are consistent with a direct association between the receptor and the membrane microdomains.
Keywords: nonhuman; animal cell; animals; cells, cultured; cell compartmentalization; embryo; calcium; neurons; microscopy, fluorescence; cholesterol; molecular interaction; ganglioside gm1; f actin; actins; cytoskeleton; centrifugation, density gradient; depolymerization; membrane permeability; membrane lipid; synapses; chick embryo; lipid rafts; neural cell adhesion molecules; methyl beta cyclodextrin; octoxynol; nicotinic receptor; neurotransmission; membrane microdomains; actin cytoskeleton; synaptogenesis; caveolin; receptors, nicotinic; receptor subunit; receptor aggregation; ciliary ganglion; synapse formation; priority journal; article; latrunculin a; ganglia, parasympathetic; ciliary nerve; cholera toxin; cholera toxin b subunit; α7 nicotinic acetylcholine receptor; receptor clusters; somatic spines
Journal Title: The Journal of Neuroscience
Volume: 21
Issue: 2
ISSN: 0270-6474
Publisher: Society for Neuroscience  
Date Published: 2001-01-15
Start Page: 504
End Page: 512
Language: English
PUBMED: 11160430
PROVIDER: scopus
Notes: Export Date: 21 May 2015 -- Source: Scopus
Citation Impact
MSK Authors
  1. Juan L Bruses
    15 Bruses