CD95 signaling via ceramide-rich membrane rafts Journal Article
| Authors: | Grassmé, H.; Jekle, A.; Riehle, A.; Schwarz, H.; Berger, J.; Sandhoff, K.; Kolesnick, R.; Gulbins, E. |
| Article Title: | CD95 signaling via ceramide-rich membrane rafts |
| Abstract: | Clustering seems to be employed by many receptors for transmembrane signaling. Here, we show that acid sphingomyelinase (ASM)-released ceramide is essential for clustering of CD95. In vitro and in vivo, extracellularly orientated ceramide, released upon CD95-triggered translocation of ASM to the plasma membrane outer surface, enabled clustering of CD95 in sphingolipid-rich membrane rafts and apoptosis induction. Whereas ASM deficiency, destruction of rafts, or neutralization of surface ceramide prevented CD95 clustering and apoptosis, natural ceramide only rescued ASM-deficient cells. The data suggest CD95-mediated clustering by ceramide is prerequisite for signaling and death. |
| Keywords: | signal transduction; human cell; metabolism; cell death; cells, cultured; apoptosis; signaling; fas antigen; cell culture; cell membrane; organic acids; sphingolipids; biochemistry; enzyme localization; ceramide; ceramides; sphingomyelin phosphodiesterase; enzyme deficiency; cells; antigens, cd95; biological membranes; sphingolipid; humans; human; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 276 |
| Issue: | 23 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2001-06-08 |
| Start Page: | 20589 |
| End Page: | 20596 |
| Language: | English |
| DOI: | 10.1074/jbc.M101207200 |
| PUBMED: | 11279185 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
