WASP recruitment to the T cell: APC contact site occurs independently of Cdc42 activation Journal Article
| Authors: | Cannon, J. L.; Labno, C. M.; Bosco, G.; Seth, A.; McGavin, M. H. K.; Siminovitch, K. A.; Rosen, M. K.; Burkhardt, J. K. |
| Article Title: | WASP recruitment to the T cell: APC contact site occurs independently of Cdc42 activation |
| Abstract: | Cdc42 and WASP are critical regulators of actin polymerization whose function during T cell signaling is poorly understood. Using a novel reagent that specifically detects Cdc42-GTP in fixed cells, we found that activated Cdc42 localizes to the T cell:APC contact site in an antigen-dependent manner. TCR signaling alone was sufficient to induce localization of Cdc42-GTP, and functional Lck and Zap-70 kinases were required. WASP also localized to the T cell:APC contact site in an antigen-dependent manner. Surprisingly, WASP localization was independent of the Cdc42 binding domain but required the proline-rich domain. Our results indicate that localized WASP activation requires the integration of multiple signals: WASP is recruited via interaction with SH3 domain-containing proteins and is activated by Cdc42-GTP concentrated at the same site. |
| Keywords: | signal transduction; controlled study; unclassified drug; human cell; protein domain; protein localization; t lymphocyte; t-lymphocytes; proteins; models, biological; animal experiment; protein binding; protein tyrosine kinase; t lymphocyte receptor; receptors, antigen, t-cell; binding site; protein structure, tertiary; protein-tyrosine kinases; intercellular junctions; antigen presenting cell; antigen-presenting cells; protein kinase lck; protein kinase zap 70; zap-70 protein-tyrosine kinase; reagent; protein cdc42; jurkat cells; proline; cell contact; wiskott aldrich syndrome protein; cdc42 gtp-binding protein; lymphocyte specific protein tyrosine kinase p56(lck); wiskott-aldrich syndrome protein; humans; human; priority journal; article; cell compartmentation |
| Journal Title: | Immunity |
| Volume: | 15 |
| Issue: | 2 |
| ISSN: | 1074-7613 |
| Publisher: | Cell Press |
| Date Published: | 2001-08-01 |
| Start Page: | 249 |
| End Page: | 259 |
| Language: | English |
| DOI: | 10.1016/s1074-7613(01)00178-9 |
| PUBMED: | 11520460 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
