In utero complementation of a neural crest-derived melanocyte defect using cell directed gene transfer Journal Article
| Authors: | Dunn, K. J.; Incao, A.; Watkins-Chow, D.; Li, Y.; Pavan, W. J. |
| Article Title: | In utero complementation of a neural crest-derived melanocyte defect using cell directed gene transfer |
| Abstract: | This study describes an in utero approach for overexpressing genes in a cell-type directed manner. It uses an avian leukosis retroviral expression system coupled with a transgenic mouse line expressing the viral receptor tv-a from a tissue-specific promoter (RCAS-TVA system) (Federspiel et al., 1994, and reviewed in Fisher et al., 1999). A transgenic mouse line was generated expressing tv-a from the Dopachrome tautomerase promoter (DCT-tv-a) in embryonic melanocyte precursors (melanoblasts). RCAS virus encoding β-galactosidase (RCAS-LacZ) or tyrosinase (RCAS-Tyr) was injected in utero into embryonic day 12.5 albino (tyrosinase inactive) mouse embryos. Animals were analyzed for β-galactosidase activity or tyrosinase activity (hair pigmentation). RCAS gene expression was detected in 44% and 25% of the transgenic mice, respectively. We demonstrate the RCAS-TVA system coupled with the DCT-tv-a line of mice can be used for in utero infection. © 2001 Wiley-Liss, Inc. |
| Keywords: | signal transduction; proto-oncogene proteins; nonhuman; mouse; animals; mice; gene overexpression; melanocyte; melanocytes; embryo; cell differentiation; enzyme activity; transfection; virus receptor; avian leukosis oncovirus; gene transfer; transgenic mouse; animalia; avian leukosis virus; mus musculus; mice, transgenic; receptors, virus; aves; enzyme inactivation; recombinant proteins; beta galactosidase; neural crest; monophenol monooxygenase; wnt proteins; gene transfer techniques; tissue specificity; genetic complementation test; complementation; zebrafish proteins; avian proteins; tyrosinase; priority journal; article; rcas; martes pennanti |
| Journal Title: | Genesis |
| Volume: | 30 |
| Issue: | 2 |
| ISSN: | 1526-954X |
| Publisher: | Wiley Blackwell |
| Date Published: | 2001-06-01 |
| Start Page: | 70 |
| End Page: | 76 |
| Language: | English |
| DOI: | 10.1002/gene.1035 |
| PUBMED: | 11416866 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
