Inhibition of 15-lipoxygenase leads to delayed organelle degradation in the reticulocyte Journal Article
| Authors: | Grüllich, C.; Duvoisin, R. M.; Wiedmann, M.; Van Leyen, K. |
| Article Title: | Inhibition of 15-lipoxygenase leads to delayed organelle degradation in the reticulocyte |
| Abstract: | Mammalian cells are characterized by an endomembrane system. Nevertheless, some cells lose these membranes during their terminal differentiation, e.g. red blood cells and lens fiber cells of the eye. 15-Lipoxygenase is believed to be critical for this membrane degradation. Here we use cultivated rabbit reticulocytes in the presence or absence of a lipoxygenase inhibitor to provide further evidence for the importance of 15-lipoxygenase for the in vivo degradation of mitochondria. We find that inhibitor treatment retarded mitochondrial degradation, as shown by persistence of marker proteins and by direct visualization of mitochondria by electron microscopy. © 2001 Federation of European Biochemical Societies. |
| Keywords: | controlled study; nonhuman; animal cell; electron microscopy; mammalia; animals; enzyme inhibition; protein; in vivo study; cell differentiation; blotting, western; endoplasmic reticulum; enzyme inhibitors; cell size; erythrocyte; mitochondrion; cell organelle; cytosol; erythrocytes; organelles; cell degeneration; rabbits; intracellular membranes; rabbit; terminal differentiation; oryctolagus cuniculus; reticulocyte; red blood cell; lipoxygenase; reticulocytes; priority journal; article; arachidonate 15 lipoxygenase; arachidonate 15-lipoxygenase; organelle degradation; eicosatetraynoic acid; icosatetraenoic acid; arachidonic acids |
| Journal Title: | FEBS Letters |
| Volume: | 489 |
| Issue: | 1 |
| ISSN: | 0014-5793 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2001-01-26 |
| Start Page: | 51 |
| End Page: | 54 |
| Language: | English |
| DOI: | 10.1016/s0014-5793(01)02080-4 |
| PUBMED: | 11231012 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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