Preparation of protein extracts from yeast Journal Article
| Authors: | Dunn, B.; Wobbe, C. R. |
| Article Title: | Preparation of protein extracts from yeast |
| Abstract: | Three protocols are presented for preparing protein extracts; they differ primarily in the way the cells are broken. In the basic protocol, cells are enzymatically converted to spheroplasts, which are then lysed by a combination of osmotic shock and Dounce homogenization. A support protocol for isolating intact nuclei by differential centrifugation is also presented. An alternate protocol describes mechanical breakage of cells by vortexing in the presence of glass beads. In a second alternate protocol, growing cells are frozen immediately in liquid nitrogen and then lysed by grinding in an industrial-strength blender in the presence of liquid nitrogen. |
| Keywords: | chemistry; cell nucleus; yeast; glass; centrifugation; isolation and purification; nitrogen; fungal protein; yeasts; fungal proteins; article; spheroplast; spheroplasts |
| Journal Title: | Current Protocols in Molecular Biology |
| Volume: | 9 |
| ISSN: | 1934-3647 |
| Publisher: | John Wiley & Sons |
| Date Published: | 1990-01-01 |
| Start Page: | 13.13.1 |
| End Page: | 13.13.9 |
| Language: | English |
| PUBMED: | 18265097 |
| PROVIDER: | scopus |
| DOI: | 10.1002/0471142727.mb1313s23 |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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