Sequence-dependent enhancement of cytotoxicity produced by ecteinascidin 743 (ET-743) with doxorubicin or paclitaxel in soft tissue sarcoma cells Journal Article
| Authors: | Takahashi, N.; Li, W.; Banerjee, D.; Scotto, K. W.; Bertino, J. R. |
| Article Title: | Sequence-dependent enhancement of cytotoxicity produced by ecteinascidin 743 (ET-743) with doxorubicin or paclitaxel in soft tissue sarcoma cells |
| Abstract: | Ecteinascidin 743 (ET-743) is a potent antitumor agent from the Caribbean tunicate Ecteinascidin turbinata and is presently in clinical trials for human cancers. To better understand how ET-743 might be used clinically, the present study used SRB assays to examine the cytotoxicity resulting from combining ET-743 with three other antineoplastic agents: doxorubicin (DXR), trimetrexate, and paclitaxel in different administration schedules in two soft tissue sarcoma cell lines, HT-1080 and HS-18, in vitro. Concurrent exposure of ET-743 with DXR resulted in synergistic interactions in both cell lines. Addition of ET-743 for 24 h before DXR was the most effective cytotoxic regimen against both cell lines. Morphological study by fluorescence microscopy revealed that combination treatment of both cells with ET-743 and DXR induced apoptosis. Exposure to paclitaxel before ET-743 was also an effective regimen. These results encourage studies of the combination of ET-743 and DXR in the treatment of soft tissue sarcoma, because each of these agents have activity in this disease. |
| Keywords: | controlled study; human cell; doxorubicin; drug potentiation; antineoplastic agents; paclitaxel; flow cytometry; cell division; apoptosis; drug potency; tumor cells, cultured; sarcoma; drug synergism; soft tissue sarcoma; fluorescence microscopy; microscopy, fluorescence; drug cytotoxicity; trabectedin; inhibitory concentration 50; dioxoles; isoquinolines; trimetrexate; tetrahydroisoquinolines; humans; human; priority journal; article |
| Journal Title: | Clinical Cancer Research |
| Volume: | 7 |
| Issue: | 10 |
| ISSN: | 1078-0432 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2001-10-01 |
| Start Page: | 3251 |
| End Page: | 3257 |
| Language: | English |
| PUBMED: | 11595721 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
