Validation of tissue microarrays for immunohistochemical profiling of cancer specimens using the example of human fibroblastic tumors Journal Article


Authors: Hoos, A.; Urist, M. J.; Stojadinovic, A.; Mastorides, S.; Dudas, M. E.; Leung, D. H. Y.; Kuo, D.; Brennan, M. F.; Lewis, J. J.; Cordon-Cardo, C.
Article Title: Validation of tissue microarrays for immunohistochemical profiling of cancer specimens using the example of human fibroblastic tumors
Abstract: Tissue microarrays allow high-throughput molecular profiling of cancer specimens by immunohistochemistry. Phenotype information of sections from arrayed biopsies on a multitissue block needs to be representative of full sections, as protein expression varies throughout the entire tumor specimen. To validate the use of tissue microarrays for immunophenotyping, we studied a group of 59 fibroblastic tumors with variable protein expression patterns by immunohistochemistry for Ki-67, p53, and the retinoblastoma protein (pRB). Data on full tissue sections were compared to the results of one, two, and three 0.6-ram core biopsies per tumor on a tissue array. Ki-67 and p53 staining was read as two categories (positive or negative). Concordance for this staining between tissue arrays with triplicate cores per tumor and full sections were 96 and 98%, respectively. For pRB staining was read as three categories (high, moderate, or negative), where concordance was 91%. The use of three cores per tumor resulted in lower numbers of lost cases and lower nonconcordance with standard full sections as compared to one or two cores per tumor. Correlations between phenotypes and clinical outcome were not significantly different between full section and array-based analysis. Triplicate 0.6-ram core biopsies sampled on tissue arrays provide a reliable system for high-throughput expression profiling by immunohistochemistry when compared to standard full sections. Triplicate cores offer a higher rate of assessable cases and a lower rate of nonconcordant readings than one or two cores. Concordance of triplicate cores is high (96 to 98%) for two category distinction and decreases with the complexity of the phenotypes being analyzed (91%).
Keywords: immunohistochemistry; adolescent; adult; child; human tissue; protein expression; aged; aged, 80 and over; middle aged; major clinical study; validation process; united states; diagnostic accuracy; ki 67 antigen; ki-67 antigen; cohort studies; gene expression profiling; protein p53; fibrosarcoma; diagnostic value; oligonucleotide array sequence analysis; fibroblast; tumor suppressor protein p53; intermethod comparison; immunophenotyping; reliability; cell nucleus; retinoblastoma protein; fibromatosis, aggressive; connective tissue tumor; humans; human; priority journal; article
Journal Title: American Journal of Pathology
Volume: 158
Issue: 4
ISSN: 0002-9440
Publisher: Elsevier Science, Inc.  
Date Published: 2001-04-01
Start Page: 1245
End Page: 1251
Language: English
PUBMED: 11290542
PROVIDER: scopus
PMCID: PMC1891917
DOI: 10.1016/S0002-9440(10)64075-8
DOI/URL:
Notes: Export Date: 21 May 2015 -- Source: Scopus
Altmetric Score
MSK Authors
  1. Murray F Brennan
    790 Brennan
  2. Jonathan J Lewis
    107 Lewis
  3. Denis Heng Yan Leung
    114 Leung
  4. Axel Hoos
    28 Hoos
  5. Maria E Dudas
    51 Dudas
  6. Marshall   Urist
    9 Urist