An imaging and analysis toolset for the study of Caenorhabditis elegans neurodevelopment Conference Paper
| Authors: | Christensen, R.; Bokinsky, A.; Santella, A.; Wu, Y.; Marquina, J.; Kovacevic, I.; Kumar, A.; Winter, P.; McCreedy, E.; Mohler, W.; Bao, Z.; Colón-Ramos, D.; Shroff, H. |
| Title: | An imaging and analysis toolset for the study of Caenorhabditis elegans neurodevelopment |
| Conference Title: | Optical Methods in Developmental Biology III |
| Abstract: | How an entire nervous system develops remains mysterious. We have developed a light-sheet microscope system to examine neurodevelopment in C. elegans embryos. Our system creates overlapping light sheets from two orthogonally positioned objectives, enabling imaging from the first cell division to hatching (∼14 hours) with 350 nm isotropic resolution. We have also developed computer algorithms to computationally straighten nematode embryos, facilitating data comparison and combination from multiple animals. We plan to use these tools to create an atlas showing the position and morphology of all neurons in the developing embryo. © 2015 SPIE. |
| Keywords: | cell proliferation; biology; algorithms; caenorhabditis elegans; neurodevelopment; cell divisions; light sheet imaging; data comparisons; isotropic resolution; light sheet; microscope systems; toolsets |
| Journal Title | Proceedings of SPIE |
| Volume: | 9334 |
| Conference Dates: | 2015 Feb 7-8 |
| Conference Location: | San Francisco, CA |
| ISBN: | 0277-786X |
| Publisher: | SPIE |
| Date Published: | 2015-03-10 |
| Start Page: | 93340C |
| Language: | English |
| DOI: | 10.1117/12.2082394 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Progr. Biomed. Opt. Imaging Proc. SPIE -- Conference code: 111854 -- Export Date: 4 May 2015 -- Nufern Inc.; Physik Instrumente; ThorLabs, Inc. -- 7 February 2015 through 8 February 2015 -- Source: Scopus |
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