Free-hand ultrasound guidance permits safe and efficient minimally invasive intrathymic injections in both young and aged mice Journal Article

   

Authors:	Tuckett, A. Z.; Zakrzewski, J. L.; Li, D.; van den Brink, M. R. M.; Thornton, R. H.
Article Title:	Free-hand ultrasound guidance permits safe and efficient minimally invasive intrathymic injections in both young and aged mice
Abstract:	The goal of this study was to evaluate whether use of an aseptic free-hand approach to ultrasound-guided injection facilitates injection into the thymic gland in mice. We used this interventional radiology technique in young, aged and immunodeficient mice and found that the thymus was visible in all cases. The mean injection period was 8 seconds in young mice and 19 seconds in aged or immunodeficient mice. Injection accuracy was confirmed by intrathymic location of an injected dye or by invivo bioluminescence imaging of injected luciferase-expressing cells. Accurate intrathymic injection was confirmed in 97% of cases. No major complications were observed. We conclude that an aseptic freehand technique for ultrasound-guided intrathymic injection is safe and accurate and reduces the time required for intrathymic injections. This method facilitates large-scale experiments and injection of individual thymic lobes and is clinically relevant. © 2015 World Federation for Ultrasound in Medicine & Biology.
Keywords:	controlled study; protein expression; aged; nonhuman; t lymphocyte; accuracy; animal cell; mouse; animal tissue; radiation; luciferase; animal experiment; animal model; in vivo study; injection; radiology; thymus; echography; interventional radiology; safety; ex vivo study; mammals; minimally invasive; severe combined immunodeficiency; imaging and display; bioluminescence imaging; minimally invasive procedure; ultrasonic applications; male; female; priority journal; article; intrathymic injection; preclinical ultrasound; aged mouse; injection period; large scale experiments; ultrasound guidance; ultrasound guided
Journal Title:	Ultrasound in Medicine and Biology
Volume:	41
Issue:	4
ISSN:	0301-5629
Publisher:	Elsevier Inc.
Date Published:	2015-04-01
Start Page:	1105
End Page:	1111
Language:	English
DOI:	10.1016/j.ultrasmedbio.2014.11.011
PROVIDER:	scopus
PMCID:	PMC4346466
PUBMED:	25701534
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84926109223&partnerID=40&md5=66a3297c9ac7088fbea15889a67c1574
Notes:	Export Date: 4 May 2015 -- Source: Scopus

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