Assessment of cancer cell line representativeness using microarrays for Merkel cell carcinoma Journal Article

Authors: Daily, K.; Coxon, A.; Williams, J. S.; Lee, C. C. R.; Coit, D. G.; Busam, K. J.; Brownell, I.
Article Title: Assessment of cancer cell line representativeness using microarrays for Merkel cell carcinoma
Abstract: When using cell lines to study cancer, phenotypic similarity to the original tumor is paramount. Yet, little has been done to characterize how closely Merkel cell carcinoma (MCC) cell lines model native tumors. To determine their similarity to MCC tumor samples, we characterized MCC cell lines via gene expression microarrays. Using whole transcriptome gene expression signatures and a computational bioinformatic approach, we identified significant differences between variant cell lines (UISO, MCC13, and MCC26) and fresh frozen MCC tumors. Conversely, the classic WaGa and Mkl-1 cell lines more closely represented the global transcriptome of MCC tumors. When compared with publicly available cancer lines, WaGa and Mkl-1 cells were similar to other neuroendocrine tumors, but the variant cell lines were not. WaGa and Mkl-1 cells grown as xenografts in mice had histological and immunophenotypical features consistent with MCC, whereas UISO xenograft tumors were atypical for MCC. Spectral karyotyping and short tandem repeat analysis of the UISO cells matched the original cell line's description, ruling out contamination. Our results validate the use of transcriptome analysis to assess the cancer cell line representativeness and indicate that UISO, MCC13, and MCC26 cell lines are not representative of MCC tumors, whereas WaGa and Mkl-1 more closely model MCC. © 2015 The Society for Investigative Dermatology.
Keywords: nonhuman; animal cell; mouse; animal tissue; gene expression; tumor xenograft; histology; neuroendocrine tumor; microarray analysis; immunophenotyping; merkel cell tumor; bioinformatics; transcriptome; contamination; spectral karyotyping; short tandem repeat; priority journal; article; cancer cell line
Journal Title: Journal of Investigative Dermatology
Volume: 135
Issue: 4
ISSN: 0022-202X
Publisher: Nature Publishing Group  
Date Published: 2015-04-01
Start Page: 1138
End Page: 1146
Language: English
DOI: 10.1038/jid.2014.518
PROVIDER: scopus
PMCID: PMC4366303
PUBMED: 25521454
Notes: Export Date: 4 May 2015 -- Source: Scopus
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MSK Authors
  1. Daniel Coit
    422 Coit
  2. Klaus J Busam
    540 Busam