Prevalence of tumor-infiltrating lymphocytes and PD-L1 expression in the soft tissue sarcoma microenvironment Journal Article
| Authors: | D'Angelo, S. P.; Shoushtari, A. N.; Agaram, N. P.; Kuk, D.; Qin, L. X.; Carvajal, R. D.; Dickson, M. A.; Gounder, M.; Keohan, M. L.; Schwartz, G. K.; Tap, W. D. |
| Article Title: | Prevalence of tumor-infiltrating lymphocytes and PD-L1 expression in the soft tissue sarcoma microenvironment |
| Abstract: | The prognostic and predictive implications of programmed death-ligand 1 (PD-L1) is unknown in sarcoma. We sought to examine the immune milieu in sarcoma specimens. We evaluated PD-L1 expression by immunohistochemistry in sarcoma specimens and quantified tumor-infiltrating lymphocytes (TIL). We correlated expression with clinical parameters and outcomes. Fifty sarcoma patients treated at Memorial Sloan Kettering Cancer Center were selected. Using the DAKO PD-L1 immunohistochemistry assay and archival formalin-fixed paraffin-embedded tissue specimens; PD-L1 expression was examined. Macrophage and lymphocyte PD-L1 status was determined qualitatively. TIL was quantified. Associations between PD-L1 expression in tumor, macrophages and lymphocytes, TIL and clinical-pathological characteristics were performed. The median age was 46 years (range, 22-76), and 66% of patients were men. Tumor, lymphocyte and macrophage PD-L1 expression was noted in 12%, 30% and 58%, respectively, with the highest prevalence in gastrointestinal stromal tumors (29%). Lymphocyte and macrophage infiltration was present in 98% and 90%, respectively. There was no association between clinical features, overall survival and PD-L1 expression in tumor or immune infiltrates. Lymphocyte and macrophage infiltration is common in sarcoma, but PD-L1 tumor expression is uncommon in sarcoma with the highest frequency observed in gastrointestinal stromal tumors. There was no association between PD-L1 expression, TIL and clinicopathological features and overall survival; however, this is limited by the heterogenous patient sample and minimal death events in the studied cohort. © 2015 Elsevier Inc. |
| Keywords: | immunohistochemistry; adult; cancer survival; clinical article; controlled study; human tissue; protein expression; aged; overall survival; clinical feature; outcome assessment; follow up; transcription factor foxp3; cd8+ t lymphocyte; tumor associated leukocyte; gastrointestinal stromal tumor; tumor volume; prevalence; histology; sarcoma; immunotherapy; cd4+ t lymphocyte; soft tissue sarcoma; leiomyosarcoma; rhabdomyosarcoma; solitary fibrous tumor; spindle cell sarcoma; synovial sarcoma; lymphocytic infiltration; macrophage; cd3+ t lymphocyte; lymphocyte; malignant peripheral nerve sheath tumor; chondrosarcoma; neuroectoderm tumor; chordoma; formaldehyde; liposarcoma; desmoid tumor; paraffin; pd-1; myxosarcoma; programmed death 1 ligand 1; hemangioendothelioma; tumor microenvironment; myoepithelioma; pd-l1; human; male; female; article; cd3+,cd4+, cd8+, foxp3+; tumor infiltrating lymphocyte |
| Journal Title: | Human Pathology |
| Volume: | 46 |
| Issue: | 3 |
| ISSN: | 0046-8177 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2015-03-01 |
| Start Page: | 357 |
| End Page: | 365 |
| Language: | English |
| DOI: | 10.1016/j.humpath.2014.11.001 |
| PROVIDER: | scopus |
| PUBMED: | 25540867 |
| PMCID: | PMC5505649 |
| DOI/URL: | |
| Notes: | Export Date: 2 April 2015 -- Source: Scopus |
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