Imaging cell death with radiolabeled annexin V in an experimental model of rheumatoid arthritis Journal Article


Authors: Post, A. M.; Katsikis, P. D.; Tait, J. F.; Geaghan, S. M.; Strauss, H. W.; Blankenberg, F. G.
Article Title: Imaging cell death with radiolabeled annexin V in an experimental model of rheumatoid arthritis
Abstract: Rheumatoid arthritis is associated with chronic synovial inflammation due to the abnormal accumulation of macrophages and autoreactive T lymphocytes in joints. The autoreactive cells cause an inflammatory proapoptotic response to self-antigens resulting in eventual bone, cartilage, and soft-tissue loss and destruction. The goal of our study was to determine the timing and intensity of apoptosis in joints using Tc-99m-labeled annexin V, an in vivo marker of apoptosis, in a murine model of immune arthritis. Methods: We used Tc-99m-annexin V and autoradiography to study the extent and severity of apoptosis in the front and rear paws of DBA/1 mice with type II collagen-induced rheumatoid arthritis. Results: Compared with control values (n = 10), there was a significant (P < 0.002) nearly 3-fold increase in uptake of Tc-99m-annexin V in the front foot pads, rear toes, rear foot pads, and heels at the time of maximal extremity swelling as determined by serial caliper measurements at 4 wk after inoculation with type II bovine collagen (n = 9). The front toes had a 5- to 6-fold increase in uptake compared with control values (P < 0.001). Histologic analysis revealed only scattered rare lymphocytes in the periarticular soft tissues, without joint destruction. Dual autoradiography with I-125-bovine serum albumin as a control showed that Tc-99m-annexin V localization was specific. Treatment with methylprednisolone for 1 wk (n = 8) at 4 wk after immunization with type II collagen decreased Tc-99m-annexin V uptake by 3- to 6-fold compared with control values (P < 0.002). Conclusion: Tc-99m-annexin V can detect collagen-induced immune arthritis and its response to steroid therapy before joint destruction.
Keywords: apoptosis; rheumatoid arthritis; phagocytosis; diseases; binding; inhibition; allograft-rejection; collagen-induced arthritis; exposure; leukocytes; annexin v; radionuclide; phosphatidylserine expression
Journal Title: Journal of Nuclear Medicine
Volume: 43
Issue: 10
ISSN: 0161-5505
Publisher: Society of Nuclear Medicine  
Date Published: 2002-10-01
Start Page: 1359
End Page: 1365
Language: English
ACCESSION: WOS:000178393900018
PROVIDER: wos
PUBMED: 12368374
Notes: Article -- Source: Wos
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MSK Authors
  1. Harry W Strauss
    166 Strauss