| Abstract: |
Purpose: Human tumors are dependent on angiogenesis for growth, and vascular endothelial growth factor (VEGF) is a major regulator of this process. We aimed to study clinical utility of a recombinant humanized monoclonal anti-VEGF antibody (rhualpha-VEGF) in the treatment of the CWR22R androgen-independent xenograft model of prostate cancer. Experimental Design: rhualphaVEGF has previously shown clinical activity in several xenograft cancer models. We administered 5 mg/kg rhualpha VEGF i.p. twice weekly as a single agent and together with paclitaxel to established CWR22R xenografts. Results: rhuaVEGF inhibited established tumor growth by 85% (P<0.01 for trajectories of the average tumor volumes of the groups) at 3 weeks, but after cessation of rhuaVEGF treatment, tumor regrowth ensued. A paclitaxel dosage of 6.25 mg/kg s.c. five times/week slowed tumor growth (72% compared with controls at 3 weeks, P=0.02). The combination of paclitaxel and rhuαVEGF resulted in greater inhibition of tumor growth than that observed with either agent alone (98% growth inhibition, P=0.024 versus rhuαVEGF alone and P=0.02 versus paclitaxel alone). Paclitaxel alone had no antiangiogenic effects at the dosage studied, whereas rhuαVEGF had significant inhibition of angiogenesis, noted by microvessel density and CD34 staining. Conclusions: rhuαVEGF has cytostatic clinical activity in this androgen-independent prostate cancer xenograft model, and the addition of paclitaxel demonstrates increased clinical activity. |
