ID1 is a functional marker for intestinal stem and progenitor cells required for normal response to injury Journal Article

Authors: Zhang, N.; Yantiss, R. K.; Nam, H. S.; Chin, Y.; Zhou, X. K.; Scherl, E. J.; Bosworth, B. P.; Subbaramaiah, K.; Dannenberg, A. J.; Benezra, R.
Article Title: ID1 is a functional marker for intestinal stem and progenitor cells required for normal response to injury
Abstract: LGR5 and BMI1 mark intestinal stem cells in crypt base columnar cells and +4 position cells, respectively, but characterization of functional markers in these cell populations is limited. ID1 maintains the stem cell potential of embryonic, neural, and longterm repopulating hematopoietic stem cells. Here, we show in both human and mouse intestine that ID1 is expressed in cycling columnar cells, +4 position cells, and transit-amplifying cells in the crypt. Lineage tracing revealed ID1+ cells to be self-renewing, multipotent stem/progenitor cells that are responsible for the long-term renewal of the intestinal epithelium. Single ID1+ cells can generate long-lived organoids resembling mature intestinal epithelium. Complete knockout of Id1 or selective deletion of Id1 in intestinal epithelium or in LGR5+ stem cells sensitizes mice to chemical-induced colon injury. These experiments identify ID1 as a marker for intestinal stem/progenitor cells and demonstrate a role for ID1 in maintaining the potential for repair in response to colonic injury.
Keywords: adult; controlled study; protein expression; unclassified drug; human cell; gene deletion; nonhuman; biological marker; animal cell; mouse; animal tissue; gene; mus; inhibitor of differentiation 1; animal experiment; animal model; cell renewal; cell lineage; bmi1 protein; multipotent stem cell; intestine crypt; colon injury; intestine epithelium; intestinal stem cell; human; male; priority journal; article; id1 gene; protein lgr5
Journal Title: Stem Cell Reports
Volume: 3
Issue: 5
ISSN: 2213-6711
Publisher: Cell Press  
Date Published: 2014-11-11
Start Page: 716
End Page: 724
Language: English
DOI: 10.1016/j.stemcr.2014.09.012
PROVIDER: scopus
PMCID: PMC4235234
PUBMED: 25418719
Notes: Export Date: 2 March 2015 -- Source: Scopus
Citation Impact
MSK Authors
  1. Robert Benezra
    144 Benezra
  2. Yvette Chin
    12 Chin
  3. Ning Zhang
    3 Zhang
  4. Hyung-Song Nam
    7 Nam