Visualization of signaling pathways and cortical cytoskeleton in cytolytic and noncytolytic natural killer cell immune synapses Journal Article

  

Authors:	Vyas, Y. M.; Maniar, H.; Dupont, B.
Article Title:	Visualization of signaling pathways and cortical cytoskeleton in cytolytic and noncytolytic natural killer cell immune synapses
Abstract:	Recent applications of imaging approaches and other methods of cell biology have provided high-resolution visualization of the location of fluorescent proteins in living and fixed cells during cell-cell interactions between lymphocytes, antigen presenting cells and target cells. We review the composition and dynamics of molecular and cyto-skeletal events occurring during natural killer cell interactions with susceptible and nonsusceptible target cells. The natural killer cell immune synapse and the concomitant changes in cytoskeletal components and cytoplasmic organelles are described. The findings are compared with the observations made in Thelper cells and cytotoxic T cells. It is concluded that the cytolytic immune synapses display spatial-temporal dynamics that are accelerated as compared with Thelper cells. In addition, the cytolytic conjugates have unique characteristics relating to their effector function. Furthermore, the natural killer cell immune synapses in cytolytic and noncytolytic interactions are distinctly different and display patterns consistent with characteristic signaling pathways identified in biochemical studies of disrupted cells. The precise relationship between different stages of the natural killer cell immune synapse formation and progression in signal transduction pathways is yet to be established.
Keywords:	signal transduction; review; nonhuman; cd8 antigen; cd8+ t lymphocyte; cd8-positive t-lymphocytes; mouse; animal; electron microscopy; metabolism; animals; mice; microscopy, electron; actin; biological model; molecular dynamics; cytotoxicity; b lymphocyte; immunoregulation; b-lymphocytes; leukosialin; t lymphocyte receptor; immunology; cd4+ t lymphocyte; cd4-positive t-lymphocytes; cytotoxic t lymphocyte; natural killer cell; killer cells, natural; cytotoxicity, immunologic; molecular interaction; cd4 antigen; cell junction; actins; intercellular junctions; cytoskeleton; ultrastructure; t lymphocyte activation; cd45 antigen; microtubule organizing center; helper cell; microtubule-organizing center; receptors, immunologic; ezrin; moesin; radixin; immunoglobulin receptor; models, immunological; humans; human; priority journal
Journal Title:	Immunological Reviews
Volume:	189
ISSN:	0105-2896
Publisher:	Wiley Blackwell
Date Published:	2002-11-01
Start Page:	161
End Page:	178
Language:	English
DOI:	10.1034/j.1600-065X.2002.18914.x
PUBMED:	12445273
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-0036864628&partnerID=40&md5=ce09d9cf71ac46b049fe214358cd95ed
Notes:	Export Date: 14 November 2014 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/67889