Human herpesvirus 6B immediate-early I protein contains functional HLA-A*02, HLA-A*03, and HLA-B*07 class I restricted CD8(+) T-cell epitopes Journal Article
| Authors: | Iampietro, M.; Morissette, G.; Gravel, A.; Dubuc, I.; Rousseau, M.; Hasan, A.; O'Reilly, R. J.; Flamand, L. |
| Article Title: | Human herpesvirus 6B immediate-early I protein contains functional HLA-A*02, HLA-A*03, and HLA-B*07 class I restricted CD8(+) T-cell epitopes |
| Abstract: | Human herpesvirus 6B (HHV-6B) is a ubiquitous pathogen with frequent reactivation observed in immunocompromised patients such as BM transplant (BMT) recipients. Adoptive immunotherapy is a promising therapeutic avenue for the treatment of opportunistic infections, including herpesviruses. While T-cell immunotherapy can successfully control CMV and EBV reactivations in BMT recipients, such therapy is not available for HHV-6 infections, in part due to a lack of identified protective CD8(+) T-cell epitopes. Our goal was to identify CD8(+) T-cell viral epitopes derived from the HHV-6B immediate-early protein I and presented by common human leukocyte Ag (HLA) class I alleles including HLA-A*02, HLA-A*03, and HLA-B*07. These epitopes were functionally tested for their ability to induce CD8(+) T-cell expansion and kill HHV-6-infected autologous cells. Cross-reactivity of specific HHV-6B-expanded T cells against HHV-6A-infected cells was also confirmed for a conserved epitope presented by HLA-A*02 molecule. Our findings will help push forward the field of adoptive immunotherapy for the treatment and/or the prevention of HHV-6 reactivation in BMT recipients. |
| Keywords: | sequence; transplantation; prediction; virology; immunotherapy; lymphocytes; cytomegalovirus; specificity; disease; inhibition; epstein-barr-virus; reactivation; cd8 t cells; hhv-6; immediate-early 1 protein; infectious; nuclear antigen-1 |
| Journal Title: | European Journal of Immunology |
| Volume: | 44 |
| Issue: | 12 |
| ISSN: | 0014-2980 |
| Publisher: | Wiley V C H Verlag Gmbh |
| Date Published: | 2014-12-01 |
| Start Page: | 3573 |
| End Page: | 3584 |
| Language: | English |
| ACCESSION: | WOS:000346701200011 |
| DOI: | 10.1002/eji.201444931 |
| PROVIDER: | wos |
| PUBMED: | 25243920 |
| Notes: | Article -- Source: Wos |
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