An iron delivery pathway mediated by a lipocalin Journal Article
| Authors: | Yang, J.; Goetz, D.; Li, J. Y.; Wang, W.; Mori, K.; Setlik, D.; Du, T.; Erdjument-Bromage, H.; Tempst, P.; Strong, R.; Barasch, J. |
| Article Title: | An iron delivery pathway mediated by a lipocalin |
| Abstract: | Despite the critical need for iron in many cellular reactions, deletion of the transferrin pathway does not block organogenesis, suggesting the presence of alternative methods to deliver iron. We show that a member of the lipocalin superfamily (24p3/Ngal) delivers iron to the cytoplasm where it activates or represses iron-responsive genes. Iron unloading depends on the cycling of 24p3/Ngal through acidic endosomes, but its pH sensitivity and its subcellular targeting differed from transferrin. Indeed, during the conversion of mesenchyme into epithelia (where we discovered the protein), 24p3/Ngal and transferrin were endocytosed by different cells that characterize different stages of development, and they triggered unique responses. These studies identify an iron delivery pathway active in development and cell physiology. |
| Keywords: | controlled study; nonhuman; animal cell; animals; cell function; cell maturation; ph; time factors; animalia; gene activation; oncogene proteins; gene repression; iron; immunoblotting; epithelium cell; epithelial cells; cytoplasm; microscopy, fluorescence; crystallography, x-ray; rats; protein family; endocytosis; mesoderm; subcellular fractions; biological transport; mesenchyme; hydrogen-ion concentration; transferrin; organogenesis; iron transport; endosome; endosomes; receptors, transferrin; silver staining; acute-phase proteins; lipocalin; article |
| Journal Title: | Molecular Cell |
| Volume: | 10 |
| Issue: | 5 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 2002-11-01 |
| Start Page: | 1045 |
| End Page: | 1056 |
| Language: | English |
| DOI: | 10.1016/s1097-2765(02)00710-4 |
| PUBMED: | 12453413 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
