Inactivation of Ras function by allele-specific peptide aptamers Journal Article
| Authors: | Xu, C. W.; Luo, Z. |
| Article Title: | Inactivation of Ras function by allele-specific peptide aptamers |
| Abstract: | One challenge facing biology is the elucidation of the function of the estimated 30 000 human genes and their polymorphic variants. Reagents that affect the activity of specific genes will be useful in the dissection of cellular regulatory networks. Here, as a test case, we used a two-bait two-hybrid system to identify peptide aptamers that distinguish allelic forms of H-Ras. Some of these anti-Ras aptamters inhibit the interaction of oncogenic Ras with c-Raf1 in vitro, and abolish EGF-stimulated activation of c-Raf1 in vivo. These experiments show that the inactivation of protein function by peptide aptamers represents a viable approach to the understanding and control of signaling pathways and oncogenic missense alleles. |
| Keywords: | signal transduction; epidermal growth factor; missense mutation; raf protein; protein function; allele; genetic variability; alleles; protein interaction; peptide; amino acid sequence; molecular sequence data; peptides; ras protein; genes, ras; two hybrid system; ras; functional genomics; aptamer; two-hybrid system techniques; proto-oncogene proteins c-raf; priority journal; article; allele-specific; peptide aptamer |
| Journal Title: | Oncogene |
| Volume: | 21 |
| Issue: | 37 |
| ISSN: | 0950-9232 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2002-08-22 |
| Start Page: | 5753 |
| End Page: | 5757 |
| Language: | English |
| DOI: | 10.1038/sj.onc.1205680 |
| PUBMED: | 12173045 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
