Pharmacological characterization of β-endorphin- and dynorphin A1-17-induced feeding using G-protein α-subunit antisense probes in rats Journal Article

  


Authors: Silva, R. M.; Grossman, H. C.; Rossi, G. C.; Pasternak, G. W.; Bodnar, R. J.
Article Title: Pharmacological characterization of β-endorphin- and dynorphin A1-17-induced feeding using G-protein α-subunit antisense probes in rats
Abstract: Antisense (AS) oligodeoxynucleotides targeting G-protein α-subunits distinguish feeding responses of morphine and its metabolite, as well as nocturnal and deprivation-induced feeding. The present study examined whether feeding elicited by β-endorphin (βEND) or dynorphin A1-17 was altered by ventricularly-applied Giα1, Giα2, Giα3, Gsα, Goα, Gqα or Gx/zα AS probes, or a nonsense (NS) control. The βEND-induced feeding was reduced by the Giα1 and Gx/zα AS probes, and increased by Giα2 or Giα3 AS treatment. Dynorphin-induced feeding was attenuated by Giα1 and Goα AS treatment. Yet, Gsα or Gqα AS and NS treatments failed to alter opioid agonist-induced feeding. These data provide initial characterization of potential effector signaling pathways mediating βEND and dynorphin-induced feeding. © 2002 Elsevier Science Inc. All rights reserved.
Keywords: signal transduction; controlled study; unclassified drug; drug potentiation; nonhuman; protein function; animal; metabolism; animals; animal experiment; gtp-binding proteins; feeding behavior; drug effect; feeding; chemistry; drug mechanism; rat; protein structure, tertiary; rats; protein subunit; rats, sprague-dawley; guanine nucleotide binding protein; protein tertiary structure; sprague dawley rat; complementary dna; narcotic agent; oligonucleotides, antisense; dynorphin; beta-endorphin; antisense oligonucleotide; narcotics; male; priority journal; article; beta endorphin; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; dynorphins; β-endorphin; dynorphin a1-17; g-protein α-subunit antisense; beta endorphin derivative; beta endorphin[1-17]; dna probe
Journal Title: Peptides
Volume: 23
Issue: 6
ISSN: 0196-9781
Publisher: Elsevier Science Inc.  
Date Published: 2002-06-01
Start Page: 1101
End Page: 1106
Language: English
DOI: 10.1016/s0196-9781(02)00036-0
PUBMED: 12126737
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0035996847&partnerID=40&md5=581a43967a50fd712258f5abc96979a0
Notes: Export Date: 14 November 2014 -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Grace Rossi
    61 Rossi
  2. Gavril W Pasternak
    414 Pasternak
Related MSK Work