A site-specific low-enrichment 15N, 13C isotope-labeling approach to unambiguous NMR spectral assignments in nucleic acids Journal Article
| Authors: | Phan, A. T.; Patel, D. J. |
| Article Title: | A site-specific low-enrichment 15N, 13C isotope-labeling approach to unambiguous NMR spectral assignments in nucleic acids |
| Abstract: | We demonstrate that base and sugar protons within a DNA oligomer can be assigned unambiguously following site-specific 15N, 13C isotope incorporation at levels as low as 1% enrichment. This simple and cost-effective methodology is demonstrated on the d(GGGTTCAGG) DNA sequence, which forms a dimeric G-quadruplex containing G·G·G·G tetrads sandwiched between G·(C-A) triads. Copyright © 2002 American Chemical Society. |
| Keywords: | analytic method; molecular dynamics; dna; isotope labeling; nuclear magnetic resonance spectroscopy; hydrogen bond; carbon isotopes; nitrogen isotopes; sequence analysis, dna; nucleic acid; organophosphorus compounds; nucleic acid structure; nuclear magnetic resonance, biomolecular; carbon 13; nitrogen 15; article |
| Journal Title: | Journal of the American Chemical Society |
| Volume: | 124 |
| Issue: | 7 |
| ISSN: | 0002-7863 |
| Publisher: | American Chemical Society |
| Date Published: | 2002-02-20 |
| Start Page: | 1160 |
| End Page: | 1161 |
| Language: | English |
| DOI: | 10.1021/ja011977m |
| PUBMED: | 11841271 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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