The yeast capping enzyme represses RNA polymerase II transcription Journal Article

Authors: Myers, L. C.; Lacomis, L.; Erdjument-Bromage, H.; Tempst, P.
Article Title: The yeast capping enzyme represses RNA polymerase II transcription
Abstract: Using a highly pure transcription system derived from Saccharomyces cerevisiae, we have purified an activity in yeast whole-cell extracts that represses RNA polymerase II transcription. Mechanistic studies suggest that this repressor specifically targets transcriptional reinitiation. The two polypeptides that constitute the repressor have been identified as Ceg1p and Cet1p, the two subunits of the yeast pre-mRNA capping enzyme. A purified recombinant capping enzyme is able to reconstitute repressor activity. Cet1p is necessary for and capable of this repression. Transcriptional run-on experiments indicate that the capping enzyme also serves as a repressor in vivo. Efficient pre-mRNA capping relies on interactions between the capping enzyme and transcription apparatus. Repression by the capping enzyme suggests a bidirectional flow of information between capping and transcription.
Keywords: controlled study; unclassified drug; nonhuman; mass spectrometry; transcription initiation; protein; transcription, genetic; acid anhydride hydrolases; messenger rna; saccharomyces cerevisiae; recombinant proteins; models, genetic; yeast; saccharomyces cerevisiae proteins; repressor proteins; rna polymerase ii; gene expression regulation, fungal; rna transcription; repressor gene; nucleotidyltransferases; chromatography, ion exchange; article; protein ceg1p; protein cet1p
Journal Title: Molecular Cell
Volume: 10
Issue: 4
ISSN: 1097-2765
Publisher: Cell Press  
Date Published: 2002-10-01
Start Page: 883
End Page: 894
Language: English
DOI: 10.1016/s1097-2765(02)00644-5
PUBMED: 12419231
PROVIDER: scopus
Notes: Export Date: 14 November 2014 -- Source: Scopus
Citation Impact
MSK Authors
  1. Paul J Tempst
    324 Tempst
  2. Lynne M Lacomis
    21 Lacomis