Activation of p53 by protein inhibitor of activated Stat1 (PIAS1) Journal Article
| Authors: | Megidish, T.; Xu, J. H.; Xu, C. W. |
| Article Title: | Activation of p53 by protein inhibitor of activated Stat1 (PIAS1) |
| Abstract: | The tumor suppressor protein p53 functions as a transcriptional factor that activates genes controlling cell cycle arrest and apoptosis. Here, we report that protein inhibitor of activated Stat1 (PIAS1) interacts with the tetramerization and C-terminal regulatory domains of p53 in yeast two-hybrid analyses. Endogenous PIAS1 is also associated with endogenous p53 in mammalian cells. Ectopic expression of PIAS1 activates p53-mediated expression in mouse embryonic fibroblast cells (p53-/-) as well as a variety of other cell lines. Furthermore, ectopic expression of PIAS1 induces p53-mediated expression of cyclin-dependent kinase inhibitor p21 and G1 arrest of the cell cycle in H1299 cells. In addition, a PIAS1 mutant without the RING-finger domain required for sumoylation could still activate p53-mediated gene expression, indicating that activation of p53 by PIAS1 does not require the RING-finger domain. Taken together, our results suggest that PIAS1 is a novel activator of p53. |
| Keywords: | controlled study; protein expression; unclassified drug; gene mutation; human cell; nonhuman; protein domain; protein function; proteins; animal cell; mammalia; animals; mice; cells, cultured; cell cycle; stat1 protein; apoptosis; genes; gene expression; carboxy terminal sequence; cell line; protein binding; gene product; gene function; protein interaction; transcription, genetic; tumor cells, cultured; hela cells; transfection; protein p53; animalia; gene activation; blotting, western; tumors; fibroblasts; tumor suppressor protein p53; plasmids; protein structure, tertiary; genes, reporter; yeast; cyclin-dependent kinase inhibitor p21; adenosine triphosphate; cyclins; cyclin dependent kinase inhibitor; luciferases; two hybrid system; protein p21; cell cycle g1 phase; g1 phase; protein inhibitor; tetramer; genes, p53; dna, complementary; mammal cell; two-hybrid system techniques; cells; mitosis inhibition; glucose-6-phosphate; precipitin tests; protein inhibitors of activated stat; humans; human; priority journal; article; protein pias1 |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 277 |
| Issue: | 10 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2002-03-08 |
| Start Page: | 8255 |
| End Page: | 8259 |
| Language: | English |
| DOI: | 10.1074/jbc.C200001200 |
| PUBMED: | 11788578 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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