High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura Journal Article
| Authors: | Levy, A. S.; Cunningham Rundles, S.; Mazza, B.; Simm, M.; Gorlick, R.; Bussel, J. |
| Article Title: | High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura |
| Abstract: | Studies have suggested that high P-glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P-glycoprotein as the likely export protein. |
| Keywords: | adolescent; adult; clinical article; controlled study; protein expression; aged; middle aged; human cell; flow cytometry; protein function; reverse transcription polymerase chain reaction; thrombocytopenia; fluorescent dyes; mononuclear cell; reverse transcriptase polymerase chain reaction; syndrome; cell culture techniques; autoimmune hemolytic anemia; idiopathic thrombocytopenic purpura; autoimmune diseases; autoimmune disease; lymphocyte; lymphocytes; p-glycoprotein; glycoprotein p; rhodamine 123; purpura, thrombocytopenic, idiopathic; humans; human; male; female; priority journal; article; multi-drug resistance; evans' syndrome |
| Journal Title: | British Journal of Haematology |
| Volume: | 118 |
| Issue: | 3 |
| ISSN: | 0007-1048 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2002-09-01 |
| Start Page: | 836 |
| End Page: | 838 |
| Language: | English |
| DOI: | 10.1046/j.1365-2141.2002.03709.x |
| PUBMED: | 12181055 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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